Naseri Leila, Khazaei Mohammad Rasool, Khazaei Mozafar
Department of Anatomical Sciences, Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Molecular Medicine, Fertility and Infertility Research Center, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Food Biochem. 2022 Mar;46(3):e13635. doi: 10.1111/jfbc.13635. Epub 2021 Feb 8.
Polycystic ovary syndrome (PCOS) is the most common cause of infertility associated with metabolic complications. Several classes of pharmacological agents have been used to manage PCOS. These drugs have shown adverse effects. Various studies showed the bee pollen (BP) as a substance rich in phytoestrogens. This study aimed to investigate the effects of BP and metformin alone and in combination with proliferation and apoptosis of granulosa cells in the rat model of PCOS. In this experimental study, 54 Wistar rats (180-210 g), was injected 2 mg of estradiol valerate intramuscularly and six rats were considered as control. After 60 days, the rats were divided into control, sham, and experimental groups. The rats were treated with bee pollen (50, 100, and 200 mg/kg) and metformin (300 mg/kg), either individually or in combination. Ovarian histology assessment was examined by H&E staining. The serum levels of NO and TNF-α were evaluated. The expressions of P53 and Ki67 were measured by IHC. In the BP and metformin-treated PCOS group, the preantral and antral follicles increased, and cystic follicles significantly decreased (p < .01). The levels of TNF-α, NO, as well as the expressions of Ki67 were decreased in the treated groups compared to the PCOS group (p < .01). On the contrary, apoptosis increased in the groups treated with BP compared to the untreated group (p < .01). BP individually or synergistically with metformin improved the symptoms of PCOS.
多囊卵巢综合征（PCOS）是与代谢并发症相关的最常见不孕症原因。几类药物已被用于治疗PCOS。这些药物已显示出不良反应。各种研究表明蜂花粉（BP）是一种富含植物雌激素的物质。本研究旨在调查BP和二甲双胍单独及联合使用对PCOS大鼠模型颗粒细胞增殖和凋亡的影响。在这项实验研究中，对54只体重180 - 210克的Wistar大鼠肌肉注射2毫克戊酸雌二醇，6只大鼠作为对照。60天后，将大鼠分为对照组、假手术组和实验组。大鼠分别单独或联合接受蜂花粉（50、100和200毫克/千克）和二甲双胍（300毫克/千克）治疗。通过苏木精-伊红（H&E）染色检查卵巢组织学评估。评估血清一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）水平。通过免疫组织化学（IHC）测量P53和Ki67的表达。在BP和二甲双胍治疗的PCOS组中，窦前卵泡和窦卵泡增加，囊性卵泡显著减少（p < 0.01）。与PCOS组相比，治疗组中TNF-α、NO水平以及Ki67的表达降低（p < 0.01）。相反，与未治疗组相比，BP治疗组的细胞凋亡增加（p < 0.01）。BP单独或与二甲双胍协同使用可改善PCOS症状。
