Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Department of Biology, Faculty of Sciences Dhar Mehraz, Sidi Mohamed Ben Abdellah University, 30000, Fez, Morocco.
New York Medical Care for Nephrology, Queens, NY, 11418, USA.
Sci Rep. 2022 May 19;12(1):8351. doi: 10.1038/s41598-022-12086-8.
Oxidative stress plays a role in hyperoxaluria-induced kidney injury and crystallization. Bee pollen is a hive product with a high content of antioxidants. The antioxidant content and protective effect of bee pollen extract (BPE) against ethylene glycol (EG) induced crystalluria, and acute kidney injury (AKI) were investigated. The effect of BPE on the EG-induced liver injury and proteinuria was also examined. Ten groups of male Wister rats were treated daily with vehicle, cystone, BPE (100, 250, and 500 mg/kg b.wt.), and group 6-9 treated with EG, EG + BPE (100, 250, and 500 mg/kg b.wt.) and group 10 EG + cystone. The dose of EG was 0.75% v/v, and the dose of cystone was 500 mg/kg b.wt. On day 30, blood and urine samples were collected for analysis. Kidneys were removed for histopathological study. The antioxidant activity of BPE was assessed, and its total phenols and flavonoids were determined. EG significantly increased urine parameters (pH, volume, calcium, phosphorus, uric acid, and protein), blood urea, creatinine, and liver enzymes (P < 0.05). EG decreased creatinine clearance and urine magnesium and caused crystalluria. Treatment with BPE or cystone mitigates EG's effect; BPE was more potent than cystone (P < 0.05). BPE increases urine volume, sodium, and magnesium compared to the control and EG treated groups. BPE reduces proteinuria and prevents AKI, crystalluria, liver injury, and histopathological changes in the kidney tissue caused by EG. BPE might have a protective effect against EG-induced AKI, crystalluria, proteinuria, and stone deposition, most likely by its antioxidant content and activity.
氧化应激在高草酸尿症引起的肾损伤和结晶中起作用。蜂花粉是一种具有高抗氧化剂含量的蜂产品。研究了蜂花粉提取物（BPE）对乙二醇（EG）诱导的结晶尿和急性肾损伤（AKI）的抗氧化含量和保护作用。还检查了 BPE 对 EG 诱导的肝损伤和蛋白尿的作用。将 10 组雄性 Wister 大鼠每天用载体、cystone、BPE（100、250 和 500mg/kg b.wt.）和用 EG、EG+BPE（100、250 和 500mg/kg b.wt.）处理的第 6-9 组以及用 EG+cystone 处理的第 10 组治疗。EG 的剂量为 0.75%v/v，cystone 的剂量为 500mg/kg b.wt。第 30 天收集血液和尿液样本进行分析。取出肾脏进行组织病理学研究。评估 BPE 的抗氧化活性，并测定其总酚类和类黄酮含量。EG 显著增加尿液参数（pH、体积、钙、磷、尿酸和蛋白质）、血尿素、肌酐和肝酶（P<0.05）。EG 降低肌酐清除率和尿液镁并导致结晶尿。用 BPE 或 cystone 治疗可减轻 EG 的作用；BPE 比 cystone 更有效（P<0.05）。与对照组和 EG 处理组相比，BPE 增加了尿量、钠和镁。BPE 减少蛋白尿并预防 AKI、结晶尿、肝损伤和 EG 引起的肾组织组织学变化。BPE 可能通过其抗氧化含量和活性对 EG 诱导的 AKI、结晶尿、蛋白尿和结石形成具有保护作用。
