He Xinxin, Liu Ronghua, Liang Jinchang, Li Yuying, Zhao Xiuxiu, Ran Lingman, Ahmad Waqar, Zhang Xiao-Hua
College of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, PR China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004687. Epub 2021 Feb 8.
A Gram-staining-negative, strictly aerobic, long-rod shaped with no flagellum and yellow-pigmented bacterium designated strain ZXX205, was isolated from the hadal seawater at the depth of 7500 m in the Mariana Trench, Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences placed strain ZXX205 within the genus and strain ZXX205 was most closely related to KCTC 52348 and KCTC 22026 with 96.9 % and 96.6 % sequence similarity, respectively. The sequence similarities to all other type strains were 96.3 % or less, and to the type strain LMG 22492 was 94.1 %. Growth occurred in the presence of 0-9.0 % (w/v) NaCl (optimum 3.0 %), at 4-45 °C (optimum 28 °C) and pH 6.0-9.0 (optimum pH 7.5). The sole respiratory quinone was menaquinone 6 (MK-6). The dominant cellular fatty acids (>10 %) of strain ZXX205 were iso-C, iso-C G, iso-C 3-OH and iso-C. The polar lipids profile contained predominantly phosphatidylethanolamine, four glycolipids, four unidentified aminolipids and three unidentified lipids. The genomic DNA G+C content was 35.5 %. The DNA-DNA relatedness (DDH) values between strain ZXX205 and the most closely related species and were 21.1 and 20.4 %, respectively. Based on polyphasic taxonomic analysis, strain ZXX205 is considered to represent a novel species in the genus of the family , for which the name is proposed. The type strain is ZXX205 (=MCCC 1K03851=JCM 33665).
从太平洋马里亚纳海沟7500米深处的超深渊海水中分离出一株革兰氏染色阴性、严格需氧、无鞭毛的长杆状且产黄色色素的细菌，命名为菌株ZXX205。基于16S rRNA基因序列的系统发育分析将菌株ZXX205置于该属内，且菌株ZXX205与KCTC 52348和KCTC 22026亲缘关系最近，序列相似性分别为96.9%和96.6%。与所有其他模式菌株的序列相似性均为96.3%或更低，与模式菌株LMG 22492的序列相似性为94.1%。在0 - 9.0%（w/v）NaCl（最适3.0%）、4 - 45°C（最适28°C）和pH 6.0 - 9.0（最适pH 7.5）条件下可生长。唯一的呼吸醌为甲基萘醌6（MK - 6）。菌株ZXX205的主要细胞脂肪酸（>10%）为异-C、异-C G、异-C 3-OH和异-C。极性脂质谱主要包含磷脂酰乙醇胺、四种糖脂、四种未鉴定的氨基脂质和三种未鉴定的脂质。基因组DNA G + C含量为35.5%。菌株ZXX205与亲缘关系最近的物种之间的DNA - DNA杂交（DDH）值分别为21.1%和20.4%。基于多相分类分析，菌株ZXX205被认为代表了科属中的一个新物种，为此提出名称。模式菌株为ZXX205（=MCCC 1K03851=JCM 33665）。
