Sun Yue, Chen Bi-Yao, Du Zong-Jun
College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.
Antonie Van Leeuwenhoek. 2017 Nov;110(11):1445-1452. doi: 10.1007/s10482-017-0897-4. Epub 2017 Jun 12.
A Gram-stain negative, non-flagellated, facultatively anaerobic, slightly halophilic bacterium, WNB302, was isolated from a marine solar saltern in Wendeng, China (122°0'38.85″E, 36°57'56.49″N). Cells of strain WNB302 were 0.2-0.7 µm wide and 2.0-10.0 µm long, catalase- positive and oxidase-negative. Colonies were opaque, orange and approximately 1.0-2.0 mm in diameter after culture for 96 h on marine agar 2216. Growth occurs at 15-37 °C (optimum, 33-35 °C), pH 6.0-8.5 (optimum, pH 7.0-7.5), and with 0.5-7% NaCl (optimum, 2-3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WNB302 belongs to the genus Winogradskyella and is closely related to Winogradskyella exilis KCTC 32356. Strain WNB302 exhibited 96.2 and 96.0% 16S rRNA gene sequence similarities with W. exilis KCTC 32356 and W. litoriviva KCTC 23972. Average nucleotide identity (ANI) value based on draft genomes between strain WNB302 and Winogradskyella thalassocola KCTC 12221 showed a relatedness of 81.1% (with 93.7% of 16S rRNA gene sequence similarity). The major respiratory quinone of strain WNB302 was found to be MK-6, and the dominant fatty acids were found to be iso-C and iso-C G. The major polar lipids of strain WNB302 were three unidentified lipids (L1, L2 and L3), one unidentified aminolipids (AL1) and phosphatidylethanolamine (PE). The genomic DNA G+C content was 37.0 mol%. On the basis of the data presented, strain WNB302 is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella aurantia sp. nov. is proposed. The type strain is WNB302 (= KCTC 52614 = MCCC 1H00172).
从中国文登的一个海洋太阳能盐场（东经122°0'38.85″，北纬36°57'56.49″）分离出一株革兰氏染色阴性、无鞭毛、兼性厌氧、轻度嗜盐的细菌WNB302。WNB302菌株的细胞宽0.2 - 0.7 µm，长2.0 - 10.0 µm，过氧化氢酶阳性，氧化酶阴性。在2216海洋琼脂上培养96小时后，菌落不透明、橙色，直径约1.0 - 2.0 mm。该菌在15 - 37 °C（最适温度33 - 35 °C）、pH 6.0 - 8.5（最适pH 7.0 - 7.5）和0.5 - 7% NaCl（最适2 - 3% NaCl）条件下生长。基于16S rRNA基因序列的系统发育分析表明，WNB302菌株属于Winogradskyella属，与微小Winogradskyella菌KCTC 32356密切相关。WNB302菌株与微小Winogradskyella菌KCTC 32356和滨海Winogradskyella菌KCTC 23972的16S rRNA基因序列相似性分别为96.2%和96.0%。基于草图基因组的菌株WNB302与海洋Winogradskyella菌KCTC 12221之间的平均核苷酸同一性（ANI）值显示其亲缘关系为81.1%（16S rRNA基因序列相似性为93.7%）。发现WNB302菌株的主要呼吸醌为MK - 6，主要脂肪酸为异C和异C G。WNB302菌株的主要极性脂类为三种未鉴定的脂类（L1、L2和L3）一种未鉴定的氨基脂类（AL1）和磷脂酰乙醇胺（PE）。基因组DNA的G + C含量为37.0 mol%。根据所提供的数据，WNB302菌株被认为代表Winogradskyella属的一个新物种，为此提出新名称橙色Winogradskyella菌（Winogradskyella aurantia sp. nov.）。模式菌株为WNB302（= KCTC 52614 = MCCC 1H00172）。
