Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Gyeongbuk Sea Grant Institute, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):321-326. doi: 10.1099/ijs.0.041384-0. Epub 2012 Mar 16.
A novel bacterium, designated F081-2(T), isolated from seawater from Damupo beach in Pohang, Korea, was investigated using a polyphasic taxonomic approach. Cells were yellow-pigmented, strictly aerobic, motile by gliding, Gram-negative-staining and rod-shaped. The temperature, pH and NaCl ranges for growth were 4-35 °C, pH 5.5-9.5 and 1.0-5.0 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F081-2(T) belonged to a distinct lineage in the genus Winogradskyella of the family Flavobacteriaceae, sharing 93.7-98.1 % similarity with recognized members of the genus. Low levels of DNA-DNA relatedness values were found between strain F081-2(T) and Winogradskyella eximia KCTC 12219(T) (61.1 %), Winogradskyella thalassocola KCTC 12221(T) (47.0 %), Winogradskyella echinorum KCTC 22026(T) (39.3 %), Winogradskyella rapida CCUG 56098(T) (34.3 %) and Winogradskyella arenosi JCM 17633(T) (33.4 %). The major cellular fatty acids were iso-C(15 : 0) (25.3 %), iso-C(15 : 1) G (14.6 %), iso-C(17 : 0) 3-OH (9.3 %), anteiso-C(15 : 0) (7.8 %) and iso-C(15 : 0) 3-OH (7.6 %). The polar lipid profile was composed of phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid, one unidentified aminophospholipid and six unidentified lipids. The major respiratory quinone was menaquinone-6 and the DNA G+C content of the strain was 32.3 mol%. On the basis of phenotypic, phylogenetic and genotypic data, strain F081-2(T) represents a novel species within the genus Winogradskyella, for which the name Winogradskyella damuponensis sp. nov. is proposed. The type strain is F081-2(T) (=KCTC 23552(T) = JCM 17633(T)).
从韩国保宁市 Damupo 海滩的海水中分离到一株新的细菌，命名为 F081-2(T)。采用多相分类学方法对其进行了研究。细胞呈黄色素，严格需氧，可通过滑行运动，革兰氏阴性染色，呈杆状。生长的温度、pH 值和 NaCl 范围分别为 4-35°C、pH5.5-9.5 和 1.0-5.0%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 F081-2(T)属于黄杆菌科 Winogradskyella 属的一个独特谱系，与该属公认成员的相似度为 93.7-98.1%。菌株 F081-2(T)与 Winogradskyella eximia KCTC 12219(T)（61.1%）、Winogradskyella thalassocola KCTC 12221(T)（47.0%）、Winogradskyella echinorum KCTC 22026(T)（39.3%）、Winogradskyella rapida CCUG 56098(T)（34.3%）和 Winogradskyella arenosi JCM 17633(T)（33.4%）之间的 DNA-DNA 同源性值较低。主要的细胞脂肪酸为 iso-C(15:0)（25.3%）、iso-C(15:1)G（14.6%）、iso-C(17:0)3-OH（9.3%）、anteiso-C(15:0)（7.8%）和 iso-C(15:0)3-OH（7.6%）。极性脂谱由磷脂酰乙醇胺、一种未鉴定的胺脂、一种未鉴定的磷脂、一种未鉴定的氨基磷脂和六种未鉴定的脂组成。主要的呼吸醌为menaquinone-6，菌株的 DNA G+C 含量为 32.3mol%。基于表型、系统发育和基因型数据，菌株 F081-2(T)代表了 Winogradskyella 属中的一个新种，因此建议将其命名为 Winogradskyella damuponensis sp. nov.。模式菌株为 F081-2(T)=KCTC 23552(T)=JCM 17633(T)。
