人关节软骨中的透明质酸。含量及大小的年龄相关性变化。
Hyaluronic acid in human articular cartilage. Age-related changes in content and size.
作者信息
Holmes M W, Bayliss M T, Muir H
机构信息
Kennedy Institute of Rheumatology, Division of Biochemistry, Hammersmith, London, U.K.
出版信息
Biochem J. 1988 Mar 1;250(2):435-41. doi: 10.1042/bj2500435.
Abstract
Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent & Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 microgram/mg wet wt. to 2.5 micrograms/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased from Mr 2.0 x 10(6) to 3.0 x 10(5) over the same age range. 3) There was no age-related change in the size of newly-synthesized hyaluronic acid, which was of very high molecular mass, in both immature and mature cartilage. The results are consistent with an age-related decrease in proteoglycan aggregate size and suggest that modification of the hyaluronic acid chain may take place in the extracellular matrix.
摘要
采用灵敏的放射吸附测定法[劳伦特和滕布拉德（1980年）《分析生物化学》第109卷，第386 - 394页]测定人关节软骨木瓜蛋白酶消化物中透明质酸的总组织含量和分子量。1）透明质酸含量在2.5岁至86岁之间从0.5微克/毫克湿重增加到2.5微克/毫克湿重。2）在相同年龄范围内，透明质酸链大小从2.0×10⁶道尔顿降至3.0×10⁵道尔顿。3）在未成熟和成熟软骨中，新合成的透明质酸分子量非常高，其大小没有与年龄相关的变化。这些结果与蛋白聚糖聚集体大小随年龄的减少一致，并表明透明质酸链的修饰可能发生在细胞外基质中。
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