新合成的蛋白聚糖对透明质酸的亲和力可通过暴露于弱碱而增强。
The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali.
作者信息
Plaas A H, Sandy J D
出版信息
Biochem J. 1986 Feb 15;234(1):221-3. doi: 10.1042/bj2340221.
The affinities for hyaluronic acid of newly synthesized proteoglycan from post-confluent rabbit chondrocyte cultures and purified bovine proteoglycan monomer were compared. In mixtures prepared at pH 6.8 the newly synthesized proteoglycan had the lower affinity; however, in mixtures incubated at pH 8.5 for 24 h before addition of hyaluronic acid, the newly synthesized proteoglycan exhibited a markedly higher affinity than the bovine monomer. The results suggest that proteoglycan secreted without associated link protein [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] has a low affinity for hyaluronate and that this may be increased during subsequent extracellular processing.
比较了来自汇合后兔软骨细胞培养物新合成的蛋白聚糖和纯化的牛蛋白聚糖单体对透明质酸的亲和力。在pH 6.8制备的混合物中，新合成的蛋白聚糖具有较低的亲和力；然而，在添加透明质酸之前于pH 8.5孵育24小时的混合物中，新合成的蛋白聚糖表现出比牛单体明显更高的亲和力。结果表明，没有相关连接蛋白分泌的蛋白聚糖[普拉斯、桑迪和缪尔（1983年）《生物化学杂志》214卷，855 - 864页]对透明质酸盐的亲和力较低，并且这可能在随后的细胞外加工过程中增加。
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