Department of Community Medicine and School of Public Health, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Advanced Pediatric Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Pediatr. 2021 Mar;88(Suppl 1):22-27. doi: 10.1007/s12098-020-03619-y. Epub 2021 Feb 8.
To report the data of burden of rotaviral acute gastroenteritis in under-five children from two states post-introduction of the vaccine.
Children under 5 y of age hospitalized with diarrhea from the states of Haryana and Himachal Pradesh in north India were recruited in the study. Commercially available ELISA kits were used for testing rotavirus in the collected stool samples. Genotyping of the positive samples was done by reverse-transcription polymerase chain reaction.
Out of 345 samples collected, 69 (20%) were found to be positive for rotavirus by ELISA. Genotyping was done and G3P[8] (31.3%), G1P[8] (13.4%), G2P[4] (13.4%) were found to be prevalent strains. Mixed strains were also found in 19.4% stool samples.
The study highlighted the high burden of rotavirus associated diarrhea in north Indian states. The data is helpful for evaluating the impact of vaccine on the severity of acute gastroenteritis and the changing strains after the introduction of rotavirus vaccine in the Universal Immunization Program.
报告印度北部两个邦引入疫苗后 5 岁以下儿童轮状病毒急性肠胃炎负担的数据。
在印度北部的哈里亚纳邦和喜马偕尔邦，招募了因腹泻住院的 5 岁以下儿童参与本研究。收集的粪便样本采用市售 ELISA 试剂盒进行轮状病毒检测。对阳性样本进行基因分型，采用逆转录聚合酶链反应。
共收集了 345 个样本，其中 69 个（20%）通过 ELISA 检测为轮状病毒阳性。对基因分型发现，G3P[8]（31.3%）、G1P[8]（13.4%）、G2P[4]（13.4%）是主要流行株。在 19.4%的粪便样本中还发现了混合株。
本研究强调了印度北部邦轮状病毒相关性腹泻的高负担。该数据有助于评估疫苗对急性肠胃炎严重程度的影响，以及在全国免疫计划中引入轮状病毒疫苗后流行株的变化。
