Department of Medicine, Addenbrookes Hospital, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2021;2244:51-81. doi: 10.1007/978-1-0716-1111-1_4.
The extensive tropism of human cytomegalovirus (HCMV) results in the productive infection of multiple cell types within the human host. However, infection of other cell types, such as undifferentiated cells of the myeloid lineage, give rise to nonpermissive infections. This aspect has been used experimentally to model latent infection, which is known to be established in the pluripotent CD34+ hematopoietic progenitor cell population resident in the bone marrow in vivo. The absence of a tractable animal model for studies of HCMV has resulted in a number of laboratories employing experimental infection of cells in vitro to simulate both HCMV lytic and latent infection. Herein, we will focus on the techniques used in our laboratory for the isolation and use of primary cells to study aspects of HCMV latency, reactivation, and lytic infection.
人巨细胞病毒（HCMV）广泛的嗜性导致其在人类宿主内的多种细胞类型中进行有性感染。然而，对其他细胞类型的感染，如未分化的髓系细胞，会导致非允许性感染。这一方面已被用于实验模拟潜伏感染，这种感染已知是在体内骨髓中多能的 CD34+造血祖细胞群体中建立的。由于缺乏用于研究 HCMV 的可处理的动物模型，许多实验室采用体外细胞感染实验来模拟 HCMV 的裂解和潜伏感染。在此，我们将重点介绍我们实验室用于分离和使用原代细胞来研究 HCMV 潜伏、再激活和裂解感染的技术。
