Department of Microbiology & Immunology, Center for Molecular and Tumor Virology, Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center-Shreveport, Shreveport, LA, USA.
Center for Cardiovascular Diseases and Sciences, Louisiana State University Health Sciences Center-Shreveport, Shreveport, LA, USA.
Methods Mol Biol. 2021;2244:103-113. doi: 10.1007/978-1-0716-1111-1_6.
Human cytomegalovirus (HCMV) can cause severe disease in the immunocompromised. One of the hallmarks of HCMV infection of a human host is the targeted infection of peripheral blood monocytes (but not other leukocyte populations) that in turn serve as the key cell type for hematogenous dissemination and the establishment of persistence following primary infection. Monocytes are also a key cell type associated with viral reactivation and spread following viral reactivation. Because of their importance in the HCMV-host infection cycle and lifelong infection, it is critical to be able to study their infection in controlled in vitro systems in the laboratory. In this chapter, we discuss a viable protocol for harvesting fresh ex vivo blood monocytes from human donors that are pure and unactivated cells and that can be used in a research setting.
人类巨细胞病毒（HCMV）可导致免疫功能低下者发生严重疾病。HCMV 感染人体的一个显著特征是针对外周血单核细胞（而不是其他白细胞群体）的靶向感染，单核细胞反过来又是血源性传播和原发性感染后持续存在的关键细胞类型。单核细胞也是与病毒再激活后病毒再激活和传播相关的关键细胞类型。由于其在 HCMV-宿主感染周期和终身感染中的重要性，因此能够在实验室的受控体外系统中研究其感染至关重要。在本章中，我们讨论了从人类供体中收获新鲜离体血液单核细胞的可行方案，这些细胞是纯的且未激活的细胞，可用于研究。
