Genomic Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Methods Mol Biol. 2021;2244:159-197. doi: 10.1007/978-1-0716-1111-1_9.
All of the cytomegaloviruses discovered to date encode two or more genes with significant homology to G protein-coupled receptors (GPCRs). The functions of these cytomegalovirus GPCRs continue to be actively studied and it is clear that they exhibit numerous interesting functions in vitro and in vivo. In this chapter, we review the various methodologies that can be used to examine biochemical aspects of viral GPCR signaling in vitro, as well as examine the biological activity of these viral GPCRs in vitro and in vivo in virus infected cells using recombinant cytomegaloviruses.
迄今为止发现的所有巨细胞病毒都编码两个或更多具有高度同源性的 G 蛋白偶联受体 (GPCR) 的基因。这些巨细胞病毒 GPCR 的功能仍在积极研究中，很明显，它们在体外和体内表现出许多有趣的功能。在本章中，我们回顾了可用于体外研究病毒 GPCR 信号转导的生化方面的各种方法，以及使用重组巨细胞病毒在病毒感染的细胞中研究这些病毒 GPCR 的体外和体内的生物学活性。
