Department of Biology, Texas Woman's University, Denton, TX, USA.
Methods Mol Biol. 2021;2244:291-299. doi: 10.1007/978-1-0716-1111-1_15.
Since its introduction in 1971, the enzyme-linked immunosorbent assay (ELISA) has revolutionized medicine by enabling detection of both antigens and antibodies in a variety of samples. We describe here a customized sandwich ELISA developed for the detection of Human Cytomegalovirus interleukin-10 (cmvIL-10). CmvIL-10 is a virally encoded cytokine and ortholog of human interleukin 10 (hIL-10). While cmvIL-10 and hIL-10 are similar in structure and function, overall amino acid sequence identity is only 27%, resulting in antigenically distinct proteins. The cmvIL-10 ELISA is specific and does not detect hIL-10. The assay is sensitive enough to detect cmvIL-10 in both culture supernatants and patient serum. The ability to quantify cmvIL-10 levels during HCMV infection could provide valuable information about immune evasion strategies and viral control of host signaling pathways.
自 1971 年问世以来，酶联免疫吸附测定（ELISA）通过能够检测各种样本中的抗原和抗体，彻底改变了医学领域。本文介绍了一种为检测人巨细胞病毒白细胞介素 10（cmvIL-10）而开发的定制夹心 ELISA。cmvIL-10 是一种病毒编码的细胞因子，与人白细胞介素 10（hIL-10）同源。尽管 cmvIL-10 和 hIL-10 在结构和功能上相似，但总体氨基酸序列同一性仅为 27%，导致抗原性不同的蛋白质。cmvIL-10 ELISA 具有特异性，不会检测到 hIL-10。该检测法足够灵敏，可检测培养上清液和患者血清中的 cmvIL-10。在 HCMV 感染期间定量 cmvIL-10 水平的能力可以提供有关免疫逃逸策略和病毒对宿主信号通路的控制的有价值信息。
