Zhou Chao, Zhou Hanlin, Ma Xueping, Yang Huilan, Wang Ping, Wang Guodong, Zheng Lanlan, Zhang Yonghong, Liu Xiaoyun
Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU), Biotechnology Research Center, China Three Gorges University, Yichang 443002, China.
Laboratory of Medicinal Plant, Institute of Basic Medical Sciences, School of Basic Medicine, Biomedical Research Institute, Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, Shiyan 442000, China.
J Agric Food Chem. 2021 Feb 24;69(7):2337-2347. doi: 10.1021/acs.jafc.0c07035. Epub 2021 Feb 8.
Post-translational modifications of histones play an important chromatic role of a transcript activity in eukaryotes. Even though mRNA and long noncoding RNA (lncRNA) genes share similar biogenetic processes, these transcript classes may differ in many ways. However, knowledge about the crosstalk between histone methylations and the two types of sorghum genes is still ambiguous. In the present study, we reveal the genome-wide distribution of six histone modifications, namely, di- and trimethylation of H3K4 (H3K4me2 and H3K4me3), H3K27 (H3K27me2 and H3K27me3), and H3K36 (H3K36me2 and H3K36me3) in sorghum and analyze their functional relationships. Unlike other histone methylation, the codecoration of H3K4me3 and H3K36me3 is negatively associated with the production of lincRNAs in the context of active expression of mRNA genes. Our data demonstrated that H3K4me3 may act as a complementary component to H3K36me3 in the transcriptional regulatory process. Moreover, we observe that both H3K4me3 and H3K36me3 are involved in the negative-going regulation of plant lincRNA and mRNA genes. Our data provide a genome-wide landscape of histone methylation in sorghum, decrypt its reciprocity, and shed light on its transcriptional regulation roles in mRNA and lncRNA genes.
组蛋白的翻译后修饰在真核生物中对转录活性起着重要的染色质作用。尽管信使核糖核酸（mRNA）和长链非编码核糖核酸（lncRNA）基因具有相似的生物发生过程，但这些转录本类别在许多方面可能存在差异。然而，关于组蛋白甲基化与高粱这两类基因之间的相互作用的知识仍然不明确。在本研究中，我们揭示了高粱中六种组蛋白修饰的全基因组分布，即H3K4的二甲基化和三甲基化（H3K4me2和H3K4me3）、H3K27的二甲基化和三甲基化（H3K27me2和H3K27me3）以及H3K36的二甲基化和三甲基化（H3K36me2和H3K36me3），并分析了它们的功能关系。与其他组蛋白甲基化不同，在mRNA基因的活跃表达背景下，H3K4me3和H3K36me3的共修饰与长链基因间非编码核糖核酸（lincRNA）的产生呈负相关。我们的数据表明，H3K4me3可能在转录调控过程中作为H3K36me3的互补成分发挥作用。此外，我们观察到H3K4me3和H3K36me3都参与了植物lincRNA和mRNA基因的负向调控。我们的数据提供了高粱组蛋白甲基化的全基因组图谱，解读了其相互作用，并阐明了其在mRNA和lncRNA基因中的转录调控作用。
