School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China; Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China; Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, University of Jinan, Jinan 250022, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 May 5;252:119499. doi: 10.1016/j.saa.2021.119499. Epub 2021 Jan 30.
The mitochondrial viscosity measurement with the amphiphilic copolymer fluorescent probe (PP) has been successfully revealed for the first time. PP was synthesized, starting from a hydrophobic rhodamine derivative fluorophore and hydrophilic 2-hydroxyethyl acrylate (HEA) by radical polymerization, which could be used to detect mitochondrial viscosity specifically. The systematic investigation demonstrated that the fluorescence emission of PP with a deep red emission increased about 9-fold when the medium is changed from methanol to 99% glycerol, indicating high viscosity dependence. Moreover, PP could self-assemble into nanospheres with the particle size of about 140 nm in water and the nano-structure enabled PP to enter living cells quickly. Cytotoxicity test showed that the cells survival rate remained above 70% at 70 μg·mL of PP. Good biocompatibility and low cytotoxicity of PP are promising to provide a high contrast fluorescence imaging. Taken together, the results point the way to development of novel amphiphilic copolymer fluorescent probes-based the detection in solutions, physiology and pathology.
首次成功地用两亲共聚物荧光探针（PP）测量了线粒体粘度。PP 是从疏水性罗丹明衍生物荧光团和亲水性 2-羟乙基丙烯酸酯（HEA）出发，通过自由基聚合合成的，可特异性检测线粒体粘度。系统研究表明，当介质从甲醇变为 99%甘油时，具有深红色发射的 PP 的荧光发射增加了约 9 倍，表明具有高粘度依赖性。此外，PP 可以在水中自组装成约 140nm 的纳米球，纳米结构使 PP 能够快速进入活细胞。细胞毒性试验表明，当 PP 的浓度为 70μg·mL 时，细胞存活率仍保持在 70%以上。PP 具有良好的生物相容性和低细胞毒性，有望提供高对比度的荧光成像。综上所述，这些结果为开发基于新型两亲共聚物荧光探针的溶液、生理和病理检测指明了方向。
