Department of Pathophysiology, Jagiellonian University Medical College, Czysta street 18, 31-121 Cracow, Poland.
Dental Institute, Central Ambulatory, University Dental Clinic, Jagiellonian University Medical College, Montelupich street 4, 31-155 Cracow, Poland.
Biomed Pharmacother. 2021 May;137:111340. doi: 10.1016/j.biopha.2021.111340. Epub 2021 Feb 5.
The aim of the current study was to investigate the influence of low-frequency electromagnetic field (LF-EMF) exposure on viability parameters of oral mucosa keratinocytes cultured in in vitro conditions. The effect of LF-EMF stimulation on cell viability was also specified in the simultaneous presence of lipopolysaccharide (LPS) infectious agent or minocycline (Mino) anti-inflammatory agent. Viability parameters such as early-, late apoptosis and necrosis of keratinocytes were analysed by the flow cytometry method (FCM). The exposure of human oral keratinocyte cell cultures to LF-EMF acting alone or combined with LPS/minocycline agents caused changes in the percentage of cells that undergo programmed or incidental cell death. The overall obtained results are compiled in a graphical form presented in Fig. 1.
本研究旨在探讨低频电磁场 (LF-EMF) 暴露对体外培养口腔黏膜角质形成细胞生存参数的影响。还在同时存在脂多糖 (LPS) 感染剂或米诺环素 (Mino) 抗炎剂的情况下，具体说明了 LF-EMF 刺激对细胞活力的影响。通过流式细胞术 (FCM) 分析角质形成细胞的早期、晚期凋亡和坏死等生存参数。单独或联合 LPS/米诺环素药物暴露于 LF-EMF 的人口腔角质形成细胞培养物导致经历程序性或偶发性细胞死亡的细胞百分比发生变化。整体获得的结果以图 1 所示的图形形式呈现。
