Zerbinati Nicola, Sommatis Sabrina, Maccario Cristina, Capillo Maria Chiara, Di Francesco Serena, Rauso Raffaele, Protasoni Marina, D'Este Edoardo, Dalla Gasperina Daniela, Mocchi Roberto
Department of Medicine and Surgery, University of Insubria, 21100 Varese, Italy.
UB-CARE S.r.l. Spin-off University of Pavia, 27010 Pavia, Italy.
Gels. 2021 Feb 4;7(1):15. doi: 10.3390/gels7010015.
The multicomponent preparations for mesotherapy are based on the principle that skin and hair aging can be prevented by supplying the fundamental substrates for correct cellular functioning, such as nucleotides, vitamins, amino acids, and biomolecules including hyaluronic acid (HA) that promote skin hydration and several biological activities. The study provides evidence for the application of HYDRO DELUXE BIO (Matex Lab S.p.A), a biocompatible hydrogel containing not cross-linked HA, for the treatment of the scalp's skin by mesotherapy. Using an in vitro model of immortalized human keratinocytes, we studied markers involved in hair aging prevention and growth, such as inflammatory markers, angiogenesis, and oxidative damage. HYDRO DELUXE BIO showed high biocompatibility and the ability to significantly reduce the expression of the inflammation marker interleukin (IL)-8 in Tumor Necrosis Factor (TNF)-stimulated cells. Then, we evaluated angiogenesis, a pivotal event during hair growth, measuring the Vascular Endothelial Growth Factor (VEGF) expression that resulted to be significantly increased in treated cells, suggesting a pro-angiogenetic capability. A protective activity against the oxidative stress agent was showed, increasing the survival rate in treated cells. Concluding, HYDRO DELUXE BIO is suitable for treatment by mesotherapy of the scalp's skin as it modulates the expression levels of markers involved in the biorevitalization of the hair follicle.
通过提供正确细胞功能所需的基本底物，如核苷酸、维生素、氨基酸以及包括透明质酸（HA）在内的生物分子（其可促进皮肤水合作用及多种生物活性），可预防皮肤和头发老化。该研究为使用HYDRO DELUXE BIO（Matex Lab S.p.A）提供了证据，这是一种含有未交联HA的生物相容性水凝胶，可用于通过中胚层疗法治疗头皮皮肤。利用永生化人角质形成细胞的体外模型，我们研究了参与预防头发老化和生长的标志物，如炎症标志物、血管生成和氧化损伤。HYDRO DELUXE BIO显示出高生物相容性，并能够显著降低肿瘤坏死因子（TNF）刺激的细胞中炎症标志物白细胞介素（IL）-8的表达。然后，我们评估了血管生成（头发生长过程中的关键事件），测量血管内皮生长因子（VEGF）的表达，结果显示在处理过的细胞中其显著增加，表明具有促血管生成能力。其还显示出对氧化应激剂的保护活性，提高了处理过的细胞的存活率。总之，HYDRO DELUXE BIO适用于通过中胚层疗法治疗头皮皮肤，因为它可调节参与毛囊生物活化的标志物的表达水平。
