School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China.
Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300401, China.
Int J Mol Sci. 2023 May 15;24(10):8797. doi: 10.3390/ijms24108797.
Fusarium crown rot (FCR), mainly caused by , not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. is a root endophytic fungus that colonizes plant roots extensively and can effectively promote plant growth and improve plant resistance to biotic and abiotic stresses. In this study, the mechanism of FCR resistance mediated by in wheat was revealed from the phenylpropanoid metabolic pathway. The results showed that the colonization of significantly reduced the progression of wheat disease, the amount of colonization, and the content of deoxynivalenol (DON) in wheat roots. RNA-seq suggested that colonization could reduce the number of differentially expressed genes (DEGs) in the transcriptome caused by infection. The DEGs induced by the colonization of were partially enriched in phenylpropanoid biosynthesis. Transcriptome sequencing and qPCR indicated that the colonization of up-regulated the expression of genes involved in the phenylpropanoid biosynthesis pathway. The metabolome analysis indicated that the colonization of increased the metabolites' accumulation in the phenylpropanoid biosynthesis. Consistent with transcriptome and metabolomic analysis, microscopic observations showed enhanced lignin accumulation in the roots of the Piri and Piri+Fp lines, most likely contributing to the arrested infection by These results suggested that increased resistance to in wheat by inducing the phenylpropanoid pathway.
镰刀菌冠腐病（FCR）主要由 引起，不仅严重威胁小麦的产量和品质，还危及人类和牲畜的健康和安全。 是一种根内生真菌，广泛定殖于植物根部，能有效促进植物生长，提高植物对生物和非生物胁迫的抗性。本研究从苯丙烷代谢途径揭示了 介导的小麦抗 FCR 机制。结果表明， 的定殖显著降低了小麦病害的进展、 的定殖量和小麦根部脱氧雪腐镰刀菌烯醇（DON）的含量。RNA-seq 表明， 的定殖可以减少 感染引起的转录组中差异表达基因（DEGs）的数量。由 定殖诱导的 DEGs 部分富集在苯丙烷生物合成中。转录组测序和 qPCR 表明， 的定殖上调了参与苯丙烷生物合成途径的基因的表达。代谢组分析表明， 的定殖增加了苯丙烷生物合成中的代谢物积累。与转录组和代谢组分析一致，显微镜观察表明，Piri 和 Piri+Fp 系根中的木质素积累增强，这可能有助于阻止 感染。这些结果表明， 通过诱导苯丙烷途径增加了小麦对 的抗性。
