Institute of Ophthalmology, University College London, London, EC1V 9EL, UK.
Department of Medical Physics and Biomedical Engineering, University College London, London, WC1E 6BT, UK.
Sci Rep. 2021 Feb 8;11(1):3274. doi: 10.1038/s41598-021-82811-2.
Mitochondrial function declines with age and in some diseases, but we have been unable to analyze this in vivo. Here, we optically examine retinal mitochondrial function as well as choroidal oxygenation and hemodynamics in aging C57 and complement factor H (CFH) mice, proposed models of macular degeneration which suffer early retinal mitochondrial decline. In young C57s mitochondrial populations respire in coupled oscillatory behavior in cycles of ~ 8 min, which is phase linked to choroidal oscillatory hemodynamics. In aging C57s, the oscillations are less regular being ~ 14 min and more dissociated from choroidal hemodynamics. The mitochondrial oscillatory cycles are extended in CFH mice being ~ 16 min and are further dissociated from choroidal hemodynamics. Mitochondrial decline occurs before age-related changes to choroidal vasculature, hence, is the likely origin of oscillatory disruption in hemodynamics. This technology offers a non-invasive technique to detect early retinal disease and its relationship to blood oxygenation in vivo and in real time.
线粒体功能随年龄增长和某些疾病而下降，但我们一直无法在体内进行分析。在这里，我们通过光学方法检查衰老 C57 和补体因子 H (CFH) 小鼠的视网膜线粒体功能以及脉络膜氧合和血液动力学，这些是黄斑变性的模型，它们早期会出现视网膜线粒体功能下降。在年轻的 C57 中，线粒体群体以约 8 分钟的周期进行偶联的振荡呼吸，与脉络膜振荡血液动力学相位相关。在衰老的 C57 中，振荡不太规则，约为 14 分钟，与脉络膜血液动力学的关联较少。CFH 小鼠的线粒体振荡周期延长至约 16 分钟，与脉络膜血液动力学的关联进一步减少。线粒体功能下降发生在与年龄相关的脉络膜血管变化之前，因此，可能是血液动力学振荡中断的起源。这项技术提供了一种非侵入性技术，可在体内实时检测早期视网膜疾病及其与血氧的关系。
