Ankara Bilkent City Hospital Otolaryngology Department, Ankara, Turkey.
Yıldırım Beyazıt University Ear Nose and Throat Department, Ankara, Turkey.
Acta Otorhinolaryngol Ital. 2020 Dec;40(6):457-462. doi: 10.14639/0392-100X-N0996.
The aim of this study was to investigate the efficacy of N-acetylcysteine (NAC) on biofilm layers and on the course of disease in chronic otitis media.
Twenty-five rats that were induced with chronic otitis media (COM) were separated into three groups. In Group 1 (N = 18), 0.2% ciprofloxacin + 0.1% dexamethasone sodium phosphate + 0.5 mg/ml NAC solution was locally injected to the right ear of the rats; in Group 2, (N=18) 0.2% ciprofloxacin + 0.1% dexamethasone sodium phosphate was locally injected to the left ear of the rats. No treatment was applied to either ear of rats in Group 3 (N = 5). Histopathological and scanning electron microscope (SEM) evaluations were performed in all groups.
SEM revealed biofilm formation in all COM induced groups. No significant difference was seen between groups 1 and 2 in terms of suppuration levels, fibrosis, inner ear involvement, infection staging and biofilm formation (p > 0.05).
In this study, while histopathological and SEM evaluation revealed no effect of 0.5 mg/ml NAC on the biofilm layer in COM-induced rats, further studies with NAC at different concentrations are still needed on different types of experimental animals.
本研究旨在探讨 N-乙酰半胱氨酸（NAC）对慢性中耳炎生物膜层及疾病进程的疗效。
将 25 只诱导慢性中耳炎（COM）的大鼠分为三组。在第 1 组（N=18）中，将 0.2%环丙沙星+0.1%地塞米松磷酸钠+0.5mg/ml NAC 溶液局部注射到大鼠右耳；第 2 组（N=18）将 0.2%环丙沙星+0.1%地塞米松磷酸钠局部注射到大鼠左耳；第 3 组（N=5）大鼠双耳均不做任何处理。对所有组均进行组织病理学和扫描电子显微镜（SEM）评估。
SEM 显示所有 COM 诱导组均有生物膜形成。在化脓水平、纤维化、内耳受累、感染分期和生物膜形成方面，第 1 组和第 2 组之间无显著差异（p>0.05）。
在这项研究中，虽然组织病理学和 SEM 评估显示，0.5mg/ml NAC 对 COM 诱导大鼠生物膜层没有影响，但仍需要在不同类型的实验动物中，用不同浓度的 NAC 进行进一步研究。
