Göger Gamze, Allak Muhammed, Şen Ali, Göger Fatih, Tekin Mehmet, Özek Gülmira
Department of Pharmacognosy, Faculty of Pharmacy, Trakya University, Edirne, Turkey.
Faculty of Pharmacy, Trakya University, Edirne, Turkey.
Z Naturforsch C J Biosci. 2021 Feb 9;76(7-8):317-327. doi: 10.1515/znc-2020-0257. Print 2021 Jul 27.
Phytochemical profiles of essential oil (), fatty acids, and -hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of L. J. Gay (syn. L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and -caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and -glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. showed the highest antioxidant activity against DPPH and ABTS radicals with IC values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, demonstrated the highest antilipoxygenase activity with an IC value of 105.40 μg/mL, whereas, howed the best inhibition of -glucosidase with an IC value of 396.40 μg/mL in the antidiabetic activity. was effective against at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of .
对L. J. Gay（同义词L.）的精油、脂肪酸以及正己烷（CAH）、乙醚（CAD）、乙酸乙酯（CAE）和甲醇提取物（CAM）的植物化学特征进行了研究，并考察了它们的抗氧化、抗炎、抗糖尿病和抗菌活性。通过气相色谱 - 质谱联用/火焰离子化检测（GC-MS/FID）对精油进行表征，发现其中苯乙酮含量为35.8%，β-石竹烯含量为10.3%。亚油酸和油酸被发现是主要脂肪酸。通过液相色谱 - 串联质谱（LC-MS/MS）发现提取物的主要成分是5-咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、异鼠李素葡萄糖苷、槲皮素和槲皮素葡萄糖苷。通过清除二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基（ABTS）来测定提取物的抗氧化活性。此外，还测定了对脂氧合酶和α-葡萄糖苷酶的抑制作用。针对革兰氏阳性菌、革兰氏阴性菌和酵母病原体评估了抗菌活性。[提取物名称]对DPPH和ABTS自由基表现出最高的抗氧化活性，IC值分别为126.60和144.40μg/mL。在抗炎活性方面，[提取物名称]表现出最高的抗脂氧合酶活性，IC值为105.40μg/mL，而在抗糖尿病活性中，[提取物名称]对α-葡萄糖苷酶的抑制效果最佳，IC值为396.40μg/mL。[提取物名称]在最低抑菌浓度（MIC）=312.5μg/mL时对[细菌名称]有效。这是关于[植物名称]不同提取物的抗糖尿病、抗炎和抗菌活性的首次报道。
