健康犬全血栓分析系统的血栓形成分析。
Analysis of blood clotting with the total thrombus analysis system in healthy dogs.
机构信息
Departments of Joint Faculty of Veterinary Medicine, Kagoshima University Veterinary Teaching Hospital, Kagoshima University, Kagoshima, Japan.
Cooperative Department of Veterinary Medicine, Animal Medical Center, Tokyo University of Agriculture and Technology, Tokyo, Japan.
出版信息
J Vet Diagn Invest. 2021 Mar;33(2):357-361. doi: 10.1177/1040638721991862. Epub 2021 Feb 9.
To date, coagulation tests are unable to reflect in vivo coagulation status in the same system, including platelet function, fibrin clot formation, and whole blood flow. The Total Thrombus Analysis System (T-TAS), which is a microfluidic assay that simulates conditions in vivo, measures whole blood flow at defined shear rates under conditions designed to assess platelet function (PL-chip) or coagulation and fibrin clot formation (AR-chip). The T-TAS records occlusion start time, occlusion time, and area under the curve. We evaluated this test in healthy control dogs. We also investigated the effect in vivo of acetylsalicylic acid (ASA), and the effect in vitro of an anticoagulation drug (dalteparin; low-molecular-weight heparin; LMWH). The CV of the AUC of both chips was good (CVs of 6.45% [PL] and 1.57% [AR]). The inhibition of platelet function by ASA was evident in the right-shift in the PL test pressure curve. The right-shift in the AR test pressure curves showed that the administration of LMWH inhibited both platelets and the coagulation cascade. The T-TAS may be useful in the evaluation of canine blood coagulation.
迄今为止，凝血测试无法在同一系统中反映体内的凝血状态，包括血小板功能、纤维蛋白凝块形成和全血流动。全血栓分析系统（T-TAS）是一种模拟体内条件的微流控分析，在设计用于评估血小板功能（PL-chip）或凝血和纤维蛋白凝块形成（AR-chip）的条件下，以规定的剪切率测量全血流动。T-TAS 记录闭塞开始时间、闭塞时间和曲线下面积。我们在健康对照犬中评估了该测试。我们还研究了体内乙酰水杨酸（ASA）的作用，以及体外抗凝药物（达肝素；低分子量肝素；LMWH）的作用。两个芯片的 AUC 的 CV 都很好（PL 的 CV 为 6.45%，AR 的 CV 为 1.57%）。ASA 对血小板功能的抑制作用体现在 PL 测试压力曲线的右移上。AR 测试压力曲线的右移表明，给予 LMWH 抑制了血小板和凝血级联反应。T-TAS 可能有助于评估犬的血液凝固。
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