Yang Caixin, Bai Yibo, Dong Kui, Yang Jing, Lai Xin-He, Lu Shan, Zhang Gui, Cheng Yanpeng, Jin Dong, Zhang Sihui, Lv Xianglian, Huang Ying, Xu Jianguo
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province 030001, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004696. Epub 2021 Feb 9.
Four Gram-stain-positive, catalase-negative, non-spore-forming, rod-shaped bacterial strains (zg-325, zg329, dk561 and dk752) were isolated from the respiratory tract of marmot () and the faeces of Tibetan gazelle () from the Qinghai-Tibet Plateau of PR China. The results of 16S rRNA gene sequence-based phylogenetic analyses indicated that strains zg-325 and dk561 represent members of the genus , most similar to DSM 20671 and B71, respectively. The DNA G+C contents of strains zg-325 and dk561 were 71.6 and 69.3 mol%, respectively. The digital DNA-DNA hybridization values of strains zg-325 and dk561 with their most closely related species were below the 70 % threshold for species demarcation. The four strains grew best at 35 °C in air containing 5 % CO on brain heart infusion (BHI) agar with 5 % sheep blood. All four strains had Cω9 and C as the major cellular fatty acids. MK-8 and MK-9 were the major menaquinones in zg-325 while MK-10 was predominant in dk561. The major polar lipids included diphosphatidylglycerol and phosphatidylinositol. On the basis of several lines of evidence from phenotypic and phylogenetic analyses, zg-325 and dk561 represent novel species of the genus , for which the name sp. nov. and sp. nov. are proposed. The type strains are zg-325 (=GDMCC 1.1724=JCM 34091) and dk561 (=CGMCC 4.7566=JCM 33484). We also propose, on the basis of the phylogenetic results herein, the reclassification of and as comb. nov. and comb. nov., respectively.
从中国青藏高原旱獭的呼吸道和藏羚羊的粪便中分离出4株革兰氏阳性、过氧化氢酶阴性、无芽孢、杆状细菌菌株(zg - 325、zg329、dk561和dk752)。基于16S rRNA基因序列的系统发育分析结果表明,菌株zg - 325和dk561分别代表属的成员,与DSM 20671和B71最为相似。菌株zg - 325和dk561的DNA G + C含量分别为71.6 mol%和69.3 mol%。菌株zg - 325和dk561与其最密切相关物种的数字DNA - DNA杂交值低于物种划分的70%阈值。这4株菌株在含5% CO₂的空气中,于35℃在添加5%羊血的脑心浸液(BHI)琼脂上生长最佳。所有4株菌株的主要细胞脂肪酸为Cω9和C。MK - 8和MK - 9是zg - 325中的主要甲基萘醌,而MK - 10在dk561中占主导地位。主要极性脂包括双磷脂酰甘油和磷脂酰肌醇。基于表型和系统发育分析的多条证据,zg - 325和dk561代表属的新物种,为此提出新名称sp. nov.和sp. nov.。模式菌株分别为zg - 325(=GDMCC 1.1724 = JCM 34091)和dk561(=CGMCC 4.7566 = JCM 33484)。基于本文的系统发育结果,我们还提议分别将和重新分类为comb. nov.和comb. nov.。