新种，新种和新种，三种纤维素分解菌，从分离自。

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

Int J Syst Evol Microbiol. 2023 May;73(5). doi: 10.1099/ijsem.0.005909.

Six facultative anaerobic, Gram-stain-positive, oxidase-negative, rod-shaped bacteria (strains zg-B89, zg-B12, zg-Y338, zg-Y138, zg-Y908 and zg-Y766), were isolated from the intestinal contents of in Qinghai Province, PR China. The 16S rRNA gene sequence analysis showed that zg-B89 showed highest similarity to NBRC 101100 (99.5 %), zg-Y338 to DSM 20118 (98.7 %), and zg-Y908 to DSM 20109 (99.0 %). Phylogenetic and phylogenomic analysis based on 16S rRNA gene and 881 core genes revealed that these six strains formed three separate clades in the genus . Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between these three novel species and all members of the genus were below species thresholds (95-96 % for ANI and 70 % for dDDH). The DNA G+C contents of zg-B89, zg-Y338 and zg-Y908 were 73.6, 72.9 and 74.5 %, respectively. Strains zg-B89 and zg-Y908 had anteiso-C, C and anteiso-C A, and zg-Y338 had anteiso-C, C and iso-C as the main fatty acids. All novel type strains had MK-9 (H) as the predominant respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as the major polar lipids, and rhamnose, ribose and glucose as the cell-wall sugars. The peptidoglycan amino acids of zg-B89, zg-Y338 and zg-Y908 contained ornithine, alanine, glutamic acid and aspartic acid (except for zg-Y338). Based on genotypic, phenotypic, phylogenetic and biochemical properties, the six uncharacterized strains represent three novel species in the genus , for which the names sp. nov. (type strain zg-B89=GDMCC 1.2821=KCTC 49756), sp. nov. (type strain zg-Y338=GDMCC 1.2829=KCTC 49754) and sp. nov. (type strain zg-Y908=GDMCC 1.2820=KCTC 49755) are proposed, respectively.

从中国青海省的肠道内容物中分离到六株兼性厌氧、革兰氏阳性、氧化酶阴性、杆状细菌（菌株 zg-B89、zg-B12、zg-Y338、zg-Y138、zg-Y908 和 zg-Y766）。16S rRNA 基因序列分析表明，zg-B89 与 NBRC 101100（99.5%）最相似，zg-Y338 与 DSM 20118（98.7%）最相似，zg-Y908 与 DSM 20109（99.0%）最相似。基于 16S rRNA 基因和 881 个核心基因的系统发育和系统基因组分析表明，这六株菌在属中形成了三个独立的分支。这三个新种与属的所有成员的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值均低于种间阈值（ANI 为 95-96%，dDDH 为 70%）。zg-B89、zg-Y338 和 zg-Y908 的 DNA G+C 含量分别为 73.6%、72.9%和 74.5%。菌株 zg-B89 和 zg-Y908 具有 anteiso-C、C 和 anteiso-C A，而 zg-Y338 具有 anteiso-C、C 和 iso-C 作为主要脂肪酸。所有新的模式株都以 MK-9（H）作为主要的呼吸醌，以双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷作为主要极性脂，以鼠李糖、核糖和葡萄糖作为细胞壁糖。菌株 zg-B89、zg-Y338 和 zg-Y908 的肽聚糖氨基酸含有鸟氨酸、丙氨酸、谷氨酸和天冬氨酸（除 zg-Y338 外）。基于基因型、表型、系统发育和生化特性，这六株未鉴定的菌株代表属中的三个新种，分别命名为 sp. nov.（模式菌株 zg-B89=GDMCC 1.2821=KCTC 49756）、 sp. nov.（模式菌株 zg-Y338=GDMCC 1.2829=KCTC 49754）和 sp. nov.（模式菌株 zg-Y908=GDMCC 1.2820=KCTC 49755）。