Department of Laboratory Medicine, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
Department of Internal Medicine, Kaunas Hospital of Lithuanian University of Health Sciences, 47144 Kaunas, Lithuania.
Medicina (Kaunas). 2021 Feb 6;57(2):148. doi: 10.3390/medicina57020148.
: Serologic testing is a useful additional method for the diagnosis of COVID-19. It is also used for population-based seroepidemiological studies. The objective of the study was to determine SARS-CoV-2 seroprevalence in healthcare workers of Kaunas hospitals and to compare two methods for specific SARS-CoV-2 antibody testing. : A total of 432 healthcare workers in Kaunas hospitals were enrolled in this study. Each participant filled a questionnaire including questions about their demographics, contact with suspected or confirmed COVID-19, acute respiratory symptoms, and whether they contacted their general practitioner, could not come to work, or had to be hospitalized. Capillary blood was used to test for SARS-CoV-2 specific immunoglobulin G (IgG) and immunoglobulin M (IgM) a lateral flow immunoassay. Serum samples were used to test for specific IgG and IgA class immunoglobulins using semiquantitative enzyme-linked immunosorbent assay (ELISA) method. : 24.77% of study participants had direct contact with a suspected or confirmed case of COVID-19. A total of 64.81% of studied individuals had at least one symptom representing acute respiratory infection, compatible with COVID-19. Lateral flow immunoassay detected SARS-CoV-2 specific IgG class immunoglobulins in 1.16% of the tested group. Fever, cough, dyspnea, nausea, diarrhea, headache, conjunctivitis, muscle pain, and loss of smell and taste predominated in the anti-SARS-CoV-2 IgG-positive group. Using ELISA, specific IgG were detected in 1.32% of the tested samples. Diarrhea, loss of appetite, and loss of smell and taste sensations were the most predominant symptoms in anti-SARS-CoV-2 IgG-positive group. The positive percent agreement of the two testing methods was 50%, and negative percent agreement was 99.66%. : 1.16% of tested healthcare workers of Kaunas hospitals were anti-SARS-CoV-2 IgG-positive. The negative percent agreement of the lateral flow immunoassay and ELISA exceeded 99%.
血清学检测是 COVID-19 诊断的一种有用的辅助方法。它也用于基于人群的血清流行病学研究。本研究的目的是确定考纳斯医院医护人员中 SARS-CoV-2 的血清流行率，并比较两种用于特定 SARS-CoV-2 抗体检测的方法。
本研究共纳入考纳斯医院的 432 名医护人员。每位参与者填写一份问卷，内容包括他们的人口统计学信息、与疑似或确诊 COVID-19 的接触情况、急性呼吸道症状以及是否联系过他们的全科医生、无法上班或需要住院等信息。使用毛细血管血检测 SARS-CoV-2 特异性免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM），采用侧向流动免疫测定法。使用血清样本，采用半定量酶联免疫吸附试验（ELISA）方法检测特定 IgG 和 IgA 类免疫球蛋白。
研究参与者中有 24.77%与疑似或确诊 COVID-19 病例有直接接触。共有 64.81%的研究对象出现至少一种代表急性呼吸道感染的症状，与 COVID-19 相符。侧向流动免疫测定法在 1.16%的检测组中检测到 SARS-CoV-2 特异性 IgG 类免疫球蛋白。在抗 SARS-CoV-2 IgG 阳性组中，发热、咳嗽、呼吸困难、恶心、腹泻、头痛、结膜炎、肌肉疼痛以及嗅觉和味觉丧失占主导地位。使用 ELISA 检测到 1.32%的检测样本中存在特异性 IgG。在抗 SARS-CoV-2 IgG 阳性组中，腹泻、食欲不振和嗅觉味觉丧失是最主要的症状。两种检测方法的阳性符合率为 50%，阴性符合率为 99.66%。
考纳斯医院的 1.16%医护人员抗 SARS-CoV-2 IgG 呈阳性。侧向流动免疫测定法和 ELISA 的阴性符合率均超过 99%。
