Ghelli Federica, Bellisario Valeria, Squillacioti Giulia, Grignani Elena, Garzaro Giacomo, Buglisi Martina, Bergamaschi Enrico, Bono Roberto
Department of Public Health and Pediatrics, University of Turin, via Santena 5 bis, 10126, Turin, Italy.
Maugeri Scientific Clinical Institutes, Via Salvatore Maugeri, 10, 27100, Pavia, Italy.
J Occup Med Toxicol. 2021 Feb 9;16(1):4. doi: 10.1186/s12995-021-00293-4.
Many workers are exposed to wood dust (WD) and formaldehyde (FA), whose carcinogenic activity is supposed to be oxidative stress-mediated. This study aims to assess to what extent the occupational exposure to WD and FA, albeit within regulatory limits, could result in OS induction in a woodworkers' population.
The sample population consisted of 127 woodworkers from 4 factories and 111 unexposed controls. Individual exposure was assessed by personal air-samplers. Each participant enrolled in the study filled out a questionnaire and provided a urinary sample to quantify OS biomarkers, namely 15-F-IsoProstane (15-F-IsoP) and 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dGuo). The main confounding factor for OS, i.e. tobacco smoking exposure, was assessed by measuring cotinine in urine samples.
Woodworkers were exposed to significantly higher amounts of WD and FA as compared to controls (p < 0.001). Among OS biomarkers, 15-F-IsoP showed statistically significant higher values in woodworkers compared to controls (p = 0.004). A significant, positive correlation was observed between 15-F-IsoP and 8-oxo-dGuo (p = 0.005), cotinine (p = 0.05), FA (p < 0.001) and WD (p = 0.01); 8-oxo-dGuo was significantly correlated with cotinine (p = 0.001) and WD (p = 0.004). In addition, WD and FA were significantly correlated each other (p < 0.001).
The study confirms that WD and FA may induce OS in woodworkers, and highlights that even the compliance with occupational exposure limits can result in measurable biological outcomes.
许多工人接触木尘（WD）和甲醛（FA），其致癌活性被认为是由氧化应激介导的。本研究旨在评估职业性接触WD和FA（尽管在监管限值范围内）在多大程度上会导致木工人群中的氧化应激（OS）诱导。
样本人群包括来自4家工厂的127名木工和111名未接触者作为对照。通过个人空气采样器评估个体接触情况。参与研究的每位参与者填写一份问卷，并提供一份尿液样本以量化OS生物标志物，即15-F-异前列腺素（15-F-IsoP）和7,8-二氢-8-氧代-2'-脱氧鸟苷（8-氧代-dGuo）。通过测量尿液样本中的可替宁评估OS的主要混杂因素，即吸烟暴露情况。
与对照组相比，木工接触的WD和FA量显著更高（p < 0.001）。在OS生物标志物中，与对照组相比，木工的15-F-IsoP值在统计学上显著更高（p = 0.004）。观察到15-F-IsoP与8-氧代-dGuo（p = 0.005）、可替宁（p = 0.05）、FA（p < 0.001）和WD（p = 0.01）之间存在显著的正相关；8-氧代-dGuo与可替宁（p = 0.001）和WD（p = 0.004）显著相关。此外，WD和FA之间也显著相关（p < 0.001）。
该研究证实WD和FA可能在木工中诱导OS，并强调即使符合职业接触限值也可能导致可测量的生物学结果。
