Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato, Tokyo 105-8461, Japan.
Division of Regenerative Medicine, Research Center for Medical Sciences, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato, Tokyo 105-8461, Japan.
Exp Anim. 2021 May 13;70(2):257-263. doi: 10.1538/expanim.20-0142. Epub 2021 Feb 10.
A reproducible swine thoracic aortic aneurysm (TAA) model is useful for investigating new therapeutic interventions. We report a surgical method for creating a reproducible swine saccular TAA model. We used eight female swine weighing 20-25 kg (LWD; ternary species). All procedures were performed under general anesthesia and involved left thoracotomy. Following aortic cross-clamping, the thoracic aorta was surgically dissected and the media and intima were resected, and the dissection plane was extended by spreading the outer layer for aneurysmal space. Subsequently, only the adventitial layer of the aorta was sutured. At 2 weeks after these procedures, angiography and computed tomography were performed. After follow-up imaging, the model animals were euthanized. Macroscopic, histological, and immunohistological examinations were performed. All model animals survived, and a saccular TAA was confirmed by follow-up imaging in all cases. The mean length of the shorter and the longer aortic diameter after the procedure were 14.01 ± 1.0 mm and 18.35 ± 1.4 mm, respectively (P<0.001). The rate of increase in the aortic diameter was 131.7 ± 13.8%, and the mean length of aneurysmal change at thoracic aorta was 22.4 ± 1.9 mm. Histological examination revealed intimal tears and defects of elastic fibers in the media. Immunostaining revealed MMP-2 and MMP-9 expressions at the aneurysm site. We report our surgical method for creating a swine saccular TAA model. Our model animal may be useful to investigate new therapeutic interventions for aortic disease.
一种可重现的猪胸主动脉瘤（TAA）模型对于研究新的治疗干预措施非常有用。我们报告了一种创建可重现的猪囊状 TAA 模型的手术方法。我们使用了 8 只体重为 20-25 公斤的雌性猪（三元物种）。所有手术均在全身麻醉下进行，涉及左开胸术。主动脉夹闭后，对胸主动脉进行手术解剖，切除中膜和内膜，并通过扩展外层来扩大夹层平面以形成动脉瘤空间。然后，仅缝合主动脉的外膜层。在这些手术后 2 周，进行血管造影和计算机断层扫描。进行随访成像后，对模型动物进行安乐死。进行大体、组织学和免疫组织化学检查。所有模型动物均存活，并且所有情况下均通过随访成像证实了囊状 TAA 的存在。手术前后主动脉短径和长径的平均值分别为 14.01±1.0mm 和 18.35±1.4mm（P<0.001）。主动脉直径的增长率为 131.7±13.8%，胸主动脉处动脉瘤变化的平均长度为 22.4±1.9mm。组织学检查显示中膜的内膜撕裂和弹性纤维缺陷。免疫组化显示 MMP-2 和 MMP-9 在动脉瘤部位的表达。我们报告了一种创建猪囊状 TAA 模型的手术方法。我们的模型动物可能有助于研究主动脉疾病的新治疗干预措施。
