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长链非编码RNA（lncRNA）RAMS11通过CBX4复合物与Top2α结合促进前列腺癌的转移和细胞生长。

Long Non-Coding RNA (lncRNA) RAMS11 Promotes Metastatis and Cell Growth of Prostate Cancer by CBX4 Complex Binding to Top2α.

作者信息

Zheng Zhixiong, Qiu Kaiyan, Huang Weiwen

机构信息

Urology Department, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 362000, People's Republic of China.

出版信息

Cancer Manag Res. 2021 Feb 2;13:913-923. doi: 10.2147/CMAR.S270144. eCollection 2021.

DOI:10.2147/CMAR.S270144
PMID:33564266
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7866953/
Abstract

INTRODUCTION

Studies have confirmed that parts of the non-coding genes in the human genome play an important role in the pathogenesis and metastasis of prostate cancer. Among them, long non-coding RNAs (lncRNAs) are vitally involved in the biological regulation of prostate cancer. In addition, lncRNAs are closely associated with the recurrence, metastasis and prognosis of prostate cancer. However, the molecular pathogenesis of lncRNAs in regulating cell growth and metastasis of prostate cancer remains unclear. Therefore, this study was designed to explore the function and mechanism of lncRNA RAMS11 in cell growth and metastasis of prostate cancer.

METHODS

Prostate cancer and para-carcinoma tissue samples were obtained from 42 patients who were diagnosed from March 2013 to September 2014 at Quanzhou First Hospital Affiliated to Fujian Medical University. Microarray experiments and real-time polymerase chain reaction (PCR) measured the expression of lncRNA. RWPE-2, LNCap, PC3 and DU145 cells were used for an in vitro model.

RESULTS

The expression of lncRNA RAMS11 was up-regulated in prostate cancer tissue samples. LncRNA RAMS11 promoted cell growth and metastasis of prostate cancer cells. Down-regulation of lncRNA RAMS11 attenuated cell growth and metastasis of prostate cancer cells. We also demonstrated that lncRNA RAMS11 bound to CBX4 to activate expression of Top2α. LncRNA RAMS11 promoted tumor growth of prostate cancer in the mouse model. The inhibition of CBX4 attenuated the pro-cancer effects of lncRNA AMS11 in prostate cancer cells, while the activation of Top2α attenuated the anti-cancer effects of si-lncRNA RAMS11 in prostate cancer cells.

DISCUSSION

Our results indicated that lncRNA RAMS11 promoted cell growth and metastasis of prostate cancer by CBX4 complex via binding to Top2α, and might be developed for the treatment of prostate cancer.

摘要

引言

研究证实，人类基因组中的部分非编码基因在前列腺癌的发病机制和转移过程中发挥着重要作用。其中，长链非编码RNA（lncRNAs）在前列腺癌的生物学调控中至关重要。此外，lncRNAs与前列腺癌的复发、转移及预后密切相关。然而，lncRNAs在调节前列腺癌细胞生长和转移中的分子发病机制仍不清楚。因此，本研究旨在探讨lncRNA RAMS11在前列腺癌细胞生长和转移中的功能及机制。

方法

从2013年3月至2014年9月在福建医科大学附属泉州第一医院确诊的42例患者中获取前列腺癌及癌旁组织样本。通过微阵列实验和实时聚合酶链反应（PCR）检测lncRNA的表达。使用RWPE-2、LNCap、PC3和DU145细胞建立体外模型。

结果

lncRNA RAMS11在前列腺癌组织样本中的表达上调。lncRNA RAMS11促进前列腺癌细胞的生长和转移。lncRNA RAMS11表达下调可减弱前列腺癌细胞的生长和转移。我们还证明lncRNA RAMS11与CBX4结合以激活Top2α的表达。lncRNA RAMS11在小鼠模型中促进前列腺癌的肿瘤生长。抑制CBX4可减弱lncRNA AMS11在前列腺癌细胞中的促癌作用，而激活Top2α可减弱si-lncRNA RAMS11在前列腺癌细胞中的抗癌作用。

讨论

我们的结果表明，lncRNA RAMS11通过与Top2α结合的CBX4复合物促进前列腺癌细胞的生长和转移，可能可用于前列腺癌的治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/487d/7866953/4deb41771fbc/CMAR-13-913-g0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/487d/7866953/4d7bb1d04ce1/CMAR-13-913-g0005.jpg
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本文引用的文献

1
The 2019 International Society of Urological Pathology (ISUP) Consensus Conference on Grading of Prostatic Carcinoma.2019 年国际泌尿病理学会（ISUP）前列腺癌分级共识会议。
Am J Surg Pathol. 2020 Aug;44(8):e87-e99. doi: 10.1097/PAS.0000000000001497.
2
A genetic risk assessment for prostate cancer influences patients' risk perception and use of repeat PSA testing: a cross-sectional study in Danish general practice.前列腺癌的基因风险评估对患者的风险认知及重复前列腺特异抗原检测的使用情况产生影响：丹麦全科医疗的一项横断面研究
BJGP Open. 2020 Jun 23;4(2). doi: 10.3399/bjgpopen20X101039. Print 2020.
3
Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia.
新兴的基于 RNA 的治疗和诊断选择：泌尿生殖系统癌症的最新进展和未来挑战。
Int J Mol Sci. 2023 Feb 27;24(5):4601. doi: 10.3390/ijms24054601.
4
Chromobox proteins in cancer: Multifaceted functions and strategies for modulation (Review).染色盒蛋白在癌症中的作用：多功能性及调控策略（综述）。
Int J Oncol. 2023 Mar;62(3). doi: 10.3892/ijo.2023.5484. Epub 2023 Feb 3.
5
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Biomedicines. 2022 Dec 26;11(1):56. doi: 10.3390/biomedicines11010056.
6
Cancer-Associated Dysregulation of Sumo Regulators: Proteases and Ligases.癌症相关的 SUMO 调节因子失调：蛋白酶和连接酶。
Int J Mol Sci. 2022 Jul 20;23(14):8012. doi: 10.3390/ijms23148012.
7
Molecular Landscape of LncRNAs in Prostate Cancer: A focus on pathways and therapeutic targets for intervention.长链非编码 RNA 在前列腺癌中的分子特征：干预途径和治疗靶点的关注。
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8
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4
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5
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Cancer. 2020 Aug 15;126(16):3667-3673. doi: 10.1002/cncr.32957. Epub 2020 May 26.
6
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Abdom Radiol (NY). 2020 Nov;45(11):3896-3906. doi: 10.1007/s00261-020-02591-7.
7
Significance of Paneth cell-like differentiation in prostatic adenocarcinoma: a retrospective cohort study of 80 cases.前列腺腺癌中潘氏细胞样分化的意义：一项对80例病例的回顾性队列研究
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8
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Anticancer Agents Med Chem. 2020;20(13):1604-1612. doi: 10.2174/1871520620666200521114815.
9
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Nat Commun. 2020 May 1;11(1):2156. doi: 10.1038/s41467-020-15547-8.
10
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EBioMedicine. 2020 Mar;53:102692. doi: 10.1016/j.ebiom.2020.102692. Epub 2020 Feb 26.