Wang Zheng, Xu Jianduo, Zhang Shumei, Chang Lili
Department of Gastroenterology, Shijiazhuang People's Hospital Shijiazhuang, Hebei, P. R. China.
Department of General Surgery, Shijiazhuang People's Hospital Shijiazhuang, Hebei, P. R. China.
Int J Clin Exp Pathol. 2021 Feb 1;14(2):151-169. eCollection 2021.
The cell division cycle-associated (CDCA) protein family plays an essential role in tumor progression by cell division. However, the function of each CDCA family member in hepatocellular carcinoma (HCC) is not well known. This study is to find the roles of CDCAs in the prognosis of HCC patients by using ONCOMINE, UALCAN, Human Protein Atlas, Kaplan-Meier Plotter, and cBioPortal databases. Overexpression of CDCA mRNA and protein were found to be significantly associated with individual cancer stages and tumor grades in HCC patients. Higher mRNA expressions of 6 CDCA family members were found to be significantly associated with shorter overall survival (OS) in HCC patients. Multivariate analysis showed that overexpressions of CDCA mRNA were independent prognostic factors for shorter OS in HCC patients. Moreover, a high mutation rate of CDCAs (27%) was also detected in HCC patients, and genetic alteration in CDCAs was associated with shorter overall survival (OS) and disease-free survival (DFS) in HCC patients. Finally, a functional analysis showed that CDCAs were mainly enriched in the cell cycle (hsa04110) and oocyte meiosis. Overall, these results indicated that CDCA2/3/4/5/8 could be prognostic biomarkers of survival in HCC patients.
细胞分裂周期相关（CDCA）蛋白家族通过细胞分裂在肿瘤进展中起重要作用。然而，每个CDCA家族成员在肝细胞癌（HCC）中的功能尚不清楚。本研究旨在通过使用ONCOMINE、UALCAN、人类蛋白质图谱、Kaplan-Meier Plotter和cBioPortal数据库来探究CDCA在HCC患者预后中的作用。研究发现，CDCA mRNA和蛋白的过表达与HCC患者的个体癌症分期和肿瘤分级显著相关。研究发现，6个CDCA家族成员的较高mRNA表达与HCC患者较短的总生存期（OS）显著相关。多变量分析表明，CDCA mRNA的过表达是HCC患者OS较短的独立预后因素。此外，在HCC患者中还检测到较高的CDCA突变率（27%），且CDCA的基因改变与HCC患者较短的总生存期（OS）和无病生存期（DFS）相关。最后，功能分析表明，CDCA主要富集于细胞周期（hsa04110）和卵母细胞减数分裂。总体而言，这些结果表明，CDCA2/3/4/5/8可能是HCC患者生存的预后生物标志物。
