University of Limpopo, Sovenga, South Africa.
Integr Cancer Ther. 2021 Jan-Dec;20:1534735420977684. doi: 10.1177/1534735420977684.
In this study, the potential of an n-butanol fraction from to prevent metastasis in MCF-7 breast cancer cells was investigated. The effect of the fraction on BUD-8 and MCF-7 cell viability was assessed using the MTT assay. Apoptotic cell death was analyzed by Hoechst staining assay. The antimetastatic effect of the fraction on MCF-7 cell was evaluated using the wound healing, adhesion and Boyden chamber invasion assays. Gelatin-zymography was used to assess the effect of the fraction on MMP-2 and MMP-9 activity. The expression profile of proteins implicated in metastasis and angiogenesis was determined using the human angiogenesis antibody array kit, following treatment with the fraction. BUD-8 cell viability was significantly reduced at concentrations between 300 and 500 µg/ml of the extract. In contrast, a significant reduction in cell viability was seen in MCF-7 cells treated with 400 to 500 µg/ml of the fraction. At sub-lethal concentrations (100 and 200 µg/ml) of the fraction, no nuclei morphological changes associated with apoptotic cell death were observed in MCF-7 cells. In addition, the fraction showed to have an inhibitory effect on MCF-7 cell migration, adhesion, invasiveness, and MMP-2 activity. Moreover, the fraction was seen to modulate the expression of several proteins, such as MMP-9, uPA, VEGF, and TGF-β1, playing a role in the metastasis process. This study demonstrates that the -butanol fraction of can inhibit major steps of the metastatic cascade and modulate metastasis regulatory proteins. Thus, the fraction can be considered a potential source of antimetastatic agents that could be useful in the treatment of malignant cancers.
在这项研究中，研究了 中的正丁醇馏分防止 MCF-7 乳腺癌细胞转移的潜力。通过 MTT 测定评估馏分对 BUD-8 和 MCF-7 细胞活力的影响。通过 Hoechst 染色测定分析细胞凋亡。通过划痕愈合、粘附和 Boyden 室侵袭测定评估馏分对 MCF-7 细胞的抗转移作用。使用明胶-酶谱法评估馏分对 MMP-2 和 MMP-9 活性的影响。用人类血管生成抗体阵列试剂盒测定经馏分处理后参与转移和血管生成的蛋白质的表达谱。BUD-8 细胞活力在提取物浓度为 300 至 500μg/ml 之间显著降低。相比之下，浓度为 400 至 500μg/ml 的馏分处理的 MCF-7 细胞活力显著降低。在馏分的亚致死浓度（100 和 200μg/ml）下，未观察到与 MCF-7 细胞凋亡相关的核形态变化。此外，馏分显示出对 MCF-7 细胞迁移、粘附、侵袭和 MMP-2 活性的抑制作用。此外，该馏分被认为可以调节几种蛋白质的表达，例如 MMP-9、uPA、VEGF 和 TGF-β1，它们在转移过程中起作用。这项研究表明， 中的正丁醇馏分可以抑制转移级联的主要步骤并调节转移调节蛋白。因此，馏分可以被认为是具有抗转移作用的潜在药物来源，可用于治疗恶性癌症。
