Broaddus V C, Wiener-Kronish J P, Berthiaume Y, Staub N C
Department of Medicine, University of California, San Francisco 94143-0130.
J Appl Physiol (1985). 1988 Jan;64(1):384-90. doi: 10.1152/jappl.1988.64.1.384.
The contribution of the parietal pleural lymphatics to pleural liquid and protein removal is unclear. We asked two questions. What is the rate of removal of sterile, artificial hydrothoraxes in awake sheep? What percentage is removed through parietal pleural lymphatics? Three days after the placement of a rib capsule in 18 sheep, we instilled a 10 ml/kg 1.0 g/dl autologous protein solution with labeled albumin and erythrocytes through the capsule into the pleural space. Erythrocytes were used as a marker for lymphatic flow. We measured terminal pleural liquid volume and radioactivity at periods from 2 to 48 h. In three sheep, we obtained a third volume measurement at 6 h by the volume of dilution technique. We found that hydrothorax removal could be described by a linear function with a constant rate: 0.28 +/- 0.01 ml.kg-1.h-1 (mean +/- SE) for the grouped data, and 0.20, 0.28, and 0.31 ml.kg-1.h-1 for the individual sheep. At 24 h, erythrocyte clearance was 89 +/- 16% (mean +/- SD) that of liquid and albumin clearance. We conclude that in awake sheep with large hydrothoraxes, pleural liquid and protein are removed at a rate of 0.28 +/- 0.01 ml.kg-1.h-1 (mean +/- SE) and lymphatics are responsible for at least 89% of this removal.
壁层胸膜淋巴管对胸腔积液和蛋白质清除的作用尚不清楚。我们提出了两个问题。清醒绵羊体内无菌人工胸腔积液的清除速率是多少？通过壁层胸膜淋巴管清除的比例是多少？在18只绵羊放置肋骨胶囊3天后，我们通过胶囊向胸腔内注入10 ml/kg 1.0 g/dl的自体蛋白质溶液，其中含有标记的白蛋白和红细胞。红细胞用作淋巴液流动的标志物。我们在2至48小时内测量终末胸腔积液量和放射性。在三只绵羊中，我们通过稀释体积技术在6小时时获得了第三次体积测量值。我们发现胸腔积液的清除可以用一个恒定速率的线性函数来描述：分组数据为0.28±0.01 ml·kg-1·h-1（平均值±标准误），个体绵羊分别为0.20、0.28和0.31 ml·kg-1·h-1。在24小时时，红细胞清除率为液体和白蛋白清除率的89±16%（平均值±标准差）。我们得出结论，在患有大量胸腔积液的清醒绵羊中，胸腔积液和蛋白质以0.28±0.01 ml·kg-1·h-1（平均值±标准误）的速率被清除，并且淋巴管至少负责其中89%的清除。
