Seibt Katharina Diana, Ghaffari Morteza Hosseini, Scheu Theresa, Koch Christian, Sauerwein Helga
Institute of Animal Science, Physiology Unit, University of Bonn, Katzenburgweg 7, 53115 Bonn, Germany.
Educational and Research Centre for Animal Husbandry, Hofgut Neumuehle, 67728 Münchweiler an der Alsenz, Germany.
Antioxidants (Basel). 2021 Feb 8;10(2):260. doi: 10.3390/antiox10020260.
A paradigm shift in the way of rearing heifer calves from restricted feeding and early weaning towards greater feed allowances and later weaning ages is ongoing. We aimed at characterizing the oxidative status in Holstein heifer calves fed with milk replacer (MR) at either a restrictive (RES) or a high (HIGH) level for 14 weeks. We compared two groups: HIGH (10 L MR/d, n = 18) and RES (5.7 L/d, n = 19) from day five until week 14 of life. In blood samples collected at birth, and then fortnightly from week 8-16, and in week 20, the antioxidative capacity measured as ferric reducing ability of plasma (FRAP), oxidative damage of lipids measured as thiobarbituric acid reactive substances (TBARS) and oxidative damage of proteins measured as advanced oxidation products of proteins (AOPP), free radicals measured as reactive oxidative metabolites (dROM), and the glutathione peroxidase (GSH-Px) activity, as well as leptin, adiponectin and haptoglobin were assessed. The time course of these variables during the first 20 weeks of life showed characteristic patterns; group differences were limited to adiponectin, AOPP, and FRAP. RES calves had lower growth rates, showed signs of hunger, but did not differ from HIGH in their intake of solid starter feed and in health status. This work characterizes the changes in oxidative status of dairy calves with increasing age and confirms the benefits of a high feeding plane with regard to welfare and development.
从限制饲养和早期断奶转向更大的饲料供应量和更晚的断奶年龄来饲养小母牛犊的方式正在发生范式转变。我们旨在描述在14周内以限制（RES）或高（HIGH）水平的代乳粉（MR）喂养的荷斯坦小母牛犊的氧化状态。我们比较了两组：从出生后第5天到第14周的HIGH组（10升MR/天，n = 18）和RES组（5.7升/天，n = 19）。在出生时采集的血样中，然后从第8 - 16周每两周采集一次，以及在第20周采集的血样中，评估了以血浆铁还原能力（FRAP）衡量的抗氧化能力、以硫代巴比妥酸反应性物质（TBARS）衡量的脂质氧化损伤、以蛋白质高级氧化产物（AOPP）衡量的蛋白质氧化损伤、以活性氧化代谢物（dROM）衡量的自由基，以及谷胱甘肽过氧化物酶（GSH-Px）活性，还有瘦素、脂联素和触珠蛋白。这些变量在生命的前20周的时间进程呈现出特征性模式；组间差异仅限于脂联素、AOPP和FRAP。RES组犊牛生长速度较低，有饥饿迹象，但在固体起始饲料摄入量和健康状况方面与HIGH组没有差异。这项工作描述了随着年龄增长奶牛犊氧化状态的变化，并证实了高饲养水平在福利和发育方面的益处。
