Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Naturhistorisches Museum, Augustinergasse 2, 4001 Basel, Switzerland.
Bull Entomol Res. 2021 Aug;111(4):411-419. doi: 10.1017/S0007485321000043. Epub 2021 Feb 11.
Diaphorina is a species-rich genus, native to the tropics and subtropics of the Old World, particularly of more arid regions. One of the species, Diaphorina citri, is the economically most important pest of citrus. Diaphorina species are morphologically similar which makes their identification difficult. In this study, the accuracy of DNA barcoding, using mitochondrial cytochrome c oxidase subunit 1 (COI), geometric morphometrics of the forewing and digital image processing methods were tested for identification of the three Diaphorina species: D. chobauti, D. citri and D. zygophylli. Moreover, the published COI sequences of D. citri, D. communis and D. lycii obtained from Genbank were used for cluster analyses. DNA barcodes for D. chobauti and D. zygophylli are deposited in Genbank for the first time. The results of the molecular and geometric morphometric analyses are congruent and place D. chobauti as the sister taxon of the other Diaphorina species. The geometric morphometric analysis shows that in D. zygophylli the fore margin is slightly curved proximally and sharply bent distally, while in D. chobauti and D. citri it is straight proximally and weakly bent distally. The results of digital image processing show that the distribution of the dark pattern differs consistently in the three studied species.
粉虱属是一个物种丰富的属，原产于旧大陆的热带和亚热带地区，特别是在较干旱的地区。其中一种，柑橘木虱，是柑橘的经济上最重要的害虫。粉虱属在形态上相似，这使得它们的鉴定变得困难。在这项研究中，使用线粒体细胞色素 c 氧化酶亚基 1（COI）的 DNA 条形码、前翅的几何形态计量学和数字图像处理方法来鉴定三种粉虱属：D. chobauti、D. citri 和 D. zygophylli。此外，还使用从 Genbank 获得的已发表的 D. citri、D. communis 和 D. lycii 的 COI 序列进行聚类分析。D. chobauti 和 D. zygophylli 的 DNA 条形码首次被存入 Genbank。分子和几何形态计量学分析的结果是一致的，将 D. chobauti 置于其他粉虱属的姐妹分类群中。几何形态计量学分析表明，在 D. zygophylli 中，前缘近端略微弯曲，远端急剧弯曲，而在 D. chobauti 和 D. citri 中，前缘近端笔直，远端微弱弯曲。数字图像处理的结果表明，在三种研究的物种中，暗纹的分布始终存在差异。
