Department of Mechanical Engineering, Shohadaye Hoveizeh University of Technology, 78986, Susangerd, Iran.
Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, 4838, Tehran, Iran.
Sci Rep. 2021 Feb 10;11(1):3521. doi: 10.1038/s41598-021-83186-0.
Cyanide-based blood poisoning can seriously damage fire victims and cause death if not detected quickly. Previous conventional methods require laboratory equipment, which are expensive and increase the duration of the analysis. Here, a simple origami based microfluidic device was introduced for point of need detection of blood cyanide concentration in people involved in fire. The device is made of four layers of paper. Each layer was in the size of 1 × 1 cm folded on each other. In this work, the blood sample was acidified by trichloroacetic acid to separate cyanide from methaemoglobin in the form of HCN gas. The produced gas released into borate buffer to recover free cyanide ions which interacted with the Pt complex ([Pt(p-MeCH)(phen)]) used as a receptor in this study. Optimized conditions were applied to have a suitable interaction causing the color of the receptor to change from yellow to colorless. The color changes were recorded by a smartphone, and the sensor response was calculated by the routine image analysis software. The assay was capable of determining cyanide ions at different concentrations in the range of 1.0 to 100.0 µmol L. The detection limit of these determination was equal to 0.4 µmol L. The assay responses were not affected by the interfering species. As a practical analysis, the proposed sensor was applied to determine cyanide ions in the blood sample of 20 studied fire survivors and 10 controls with high accuracy.
基于氰化物的血液中毒如果不能迅速发现，会严重损害火灾受害者的身体并导致其死亡。以前的传统方法需要实验室设备，这些设备既昂贵又会延长分析时间。在这里，我们介绍了一种简单的折纸式微流控装置，用于在现场即时检测火灾中涉及人员的血液氰化物浓度。该装置由四层纸制成，每一层的尺寸为 1×1 厘米，相互折叠。在这项工作中，血液样本用三氯乙酸酸化，以将氰化物与高铁血红蛋白分离，形成 HCN 气体。产生的气体释放到硼酸盐缓冲液中，以回收与作为本研究中受体的[Pt(p-MeCH)(phen)]相互作用的游离氰化物离子。优化条件用于进行合适的相互作用，导致受体的颜色从黄色变为无色。智能手机记录颜色变化，并用常规图像分析软件计算传感器响应。该测定法能够在 1.0 至 100.0 μmol L 的不同浓度范围内测定氰化物离子。这些测定的检测限等于 0.4 μmol L。该测定法的响应不受干扰物质的影响。作为实际分析，将所提出的传感器应用于 20 名研究火灾幸存者和 10 名对照者的血液样本中，以高精度测定氰化物离子。
