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长链非编码RNA NR2F1-AS1和长链非编码RNA H19基因在肝细胞癌中的作用及其对Huh-7细胞生物学功能的影响

Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7.

作者信息

Ji Wen-Chao, Bao Guang-Jian, Yang Fang-Wu, Sun Lei, Han Rui

机构信息

Department of Hepatobiliary Surgery, Zaozhuang Municipal Hospital, Zaozhuang, 277100 Shandong Province, People's Republic of China.

General Surgery Department, Zaozhuang Mining Group Central Hospital, Zaozhuang, 277100 Shandong Province, People's Republic of China.

出版信息

Cancer Manag Res. 2021 Feb 3;13:941-951. doi: 10.2147/CMAR.S284650. eCollection 2021.

DOI:10.2147/CMAR.S284650
PMID:33568940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7868256/
Abstract

OBJECTIVE

This research was designed to probe into the expression and related mechanism of lncRNA NR2F1-AS1 and H19 in hepatocellular carcinoma (HCC).

METHODS

Forty-two HCC patients who came to our hospital from February 2018 to August 2019 were included into a research group (RG). Meanwhile, 46 healthy controls were regarded as a control group (CG). BEL-7402, Huh-7 human hepatoma cells and HL-7702 human normal liver cells were purchased, and the NR2F1-AS1 and H19 levels in serum and tissues of HCC patients were detected. PcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19 and si-H19 were transfected into BEL-7402 and Huh-7 cells. The NR2F1-AS1 and H19 levels in samples were detected via qRT-PCR, and the expression of apoptosis-related proteins in cells was tested through WB. Cell proliferation, invasion, or apoptosis was detected by CCK8, Transwell or flow cytometry, respectively.

RESULTS

The NR2F1-AS1 and H19 levels were high in human hepatoma cells, and AUCs of lncRNA NR2F1-AS1 and lncRNA H19 were both >0.8. The lncRNA NR2F1-AS1 and lncRNA H19 were associated with HCC staging. After transfection of pcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19, si-H19 BEL-7402 and Huh-7 cells, silencing NR2F1-AS1 and H19 expression can promote apoptosis and inhibit cell growth, while silencing their over-expression can inhibit the EMT process of Huh-7 cells.

CONCLUSION

lncRNA NR2F1-AS1 and lncRNA H19 genes are abnormally expressed in HCC. Furthermore, the two can suppress the EMT process of Huh-7 cells and promote apoptosis effectively.

摘要

目的

本研究旨在探讨长链非编码RNA（lncRNA）NR2F1-AS1和H19在肝细胞癌（HCC）中的表达及相关机制。

方法

选取2018年2月至2019年8月来我院就诊的42例HCC患者作为研究组（RG）。同时，将46例健康对照者作为对照组（CG）。购买BEL-7402、Huh-7人肝癌细胞和HL-7702人正常肝细胞，检测HCC患者血清和组织中NR2F1-AS1和H19水平。将PcDNA3.1-NR2F1-AS1、si-NR2F1-AS1、NC、pcDNA3.1-H19和si-H19转染至BEL-7402和Huh-7细胞。通过qRT-PCR检测样本中NR2F1-AS1和H19水平，通过蛋白质免疫印迹法（WB）检测细胞中凋亡相关蛋白的表达。分别采用CCK8、Transwell或流式细胞术检测细胞增殖、侵袭或凋亡情况。

结果

人肝癌细胞中NR2F1-AS1和H19水平较高，lncRNA NR2F1-AS1和lncRNA H19的曲线下面积（AUC）均>0.8。lncRNA NR2F1-AS1和lncRNA H19与HCC分期相关。将PcDNA3.1-NR2F1-AS1、si-NR2F1-AS1、NC、pcDNA3.1-H19、si-H19转染至BEL-7402和Huh-7细胞后，沉默NR2F1-AS1和H19表达可促进细胞凋亡并抑制细胞生长，而沉默其过表达可抑制Huh-7细胞的上皮-间质转化（EMT）过程。

结论

lncRNA NR2F1-AS1和lncRNA H19基因在HCC中异常表达。此外，二者可有效抑制Huh-7细胞的EMT过程并促进细胞凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/dcedebf6fdce/CMAR-13-941-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/cd850dc95fe0/CMAR-13-941-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/aea9664edd39/CMAR-13-941-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/1083be0b9ce0/CMAR-13-941-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/4e41045bb136/CMAR-13-941-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/4fec1acd8d02/CMAR-13-941-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/adc2dccaa12c/CMAR-13-941-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/dcedebf6fdce/CMAR-13-941-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/cd850dc95fe0/CMAR-13-941-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/aea9664edd39/CMAR-13-941-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/1083be0b9ce0/CMAR-13-941-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/4e41045bb136/CMAR-13-941-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/4fec1acd8d02/CMAR-13-941-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/adc2dccaa12c/CMAR-13-941-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daf7/7868256/dcedebf6fdce/CMAR-13-941-g0007.jpg

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2
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3
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4
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