Department of Health Care, Jinan Central Hospital, Jinan, Shandong, China.
Department of Spleen and Stomach Diseases, Liaocheng Chinese Medicine Hospital, Liaocheng, Shandong, China.
Bioengineered. 2021 Dec;12(2):12345-12356. doi: 10.1080/21655979.2021.2001168.
Gastric cancer (GC) is a highly malignant solid tumor of the digestive tract, which is associated with a high mortality rate. Long non-coding RNA (lncRNA) nuclear receptor subfamily 2 group F member 1 antisense RNA 1 (NR2F1-AS1) has been reported to exert a tumor-promoting effect in some types of cancer. The present study aimed to investigate the role of NR2F1-AS1 in GC. The expression levels of NR2F1-AS1 and its potential target gene were measured in GC cell lines. Bioinformatics analysis, an RNA immunoprecipitation assay and a chromatin immunoprecipitation assay were used to determine the binding relationship between NR2F1-AS1 and downstream genes. The effect of NR2F1-AS1 regulatory axis on AGC cell viability, proliferation, migration, invasion and epithelial-mesenchymal transition was evaluated. The results of the present study revealed that the knockdown of NR2F1-AS1 inhibited the proliferation, invasion and migration of GC cells. NR2F1-AS1 also upregulated the expression levels of ST8SIA1 by recruiting transcriptional factor SPI1. Thus, the effects of the knockdown of NR2F1-AS1 on GC cell functions were suggested to occur via regulation of ST8SIA1. In conclusion, the findings of the current study indicated that NR2F1-AS1 may promote the proliferation, invasion and migration of GC cells by recruiting SPI1, to upregulate ST8SIA1 expression. Thus, the regulation of their expression levels may provide a novel direction for the treatment of GC.
胃癌（GC）是一种高度恶性的消化道实体瘤，其死亡率较高。长链非编码 RNA（lncRNA）核受体亚家族 2 组 F 成员 1 反义 RNA 1（NR2F1-AS1）已被报道在某些类型的癌症中发挥促肿瘤作用。本研究旨在探讨 NR2F1-AS1 在 GC 中的作用。测量了 GC 细胞系中 NR2F1-AS1及其潜在靶基因的表达水平。通过生物信息学分析、RNA 免疫沉淀测定和染色质免疫沉淀测定来确定 NR2F1-AS1 与下游基因之间的结合关系。评估了 NR2F1-AS1 调节轴对 AGC 细胞活力、增殖、迁移、侵袭和上皮-间充质转化的影响。本研究的结果表明，NR2F1-AS1 的敲低抑制了 GC 细胞的增殖、侵袭和迁移。NR2F1-AS1 还通过募集转录因子 SPI1 上调了 ST8SIA1 的表达水平。因此，NR2F1-AS1 敲低对 GC 细胞功能的影响可能是通过调节 ST8SIA1 发生的。综上所述，本研究结果表明，NR2F1-AS1 可能通过募集 SPI1 促进 GC 细胞的增殖、侵袭和迁移，上调 ST8SIA1 的表达。因此，调节它们的表达水平可能为 GC 的治疗提供新的方向。
