Huang Xing, Liang Yongsheng, Zhang Baoqing, Song Xiupeng, Li Yangrui, Qin Zhengqiang, Li Dewei, Chen Rongfa, Zhou Zhongfeng, Deng Yuchi, Wei Jiguang, Wu Jianming
College of Agriculture, Guangxi University, Nanning, China.
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Ministry of Agriculture, Nanning, China.
Front Genet. 2021 Jan 25;11:581993. doi: 10.3389/fgene.2020.581993. eCollection 2020.
Cold stress causes major losses to sugarcane production, yet the precise molecular mechanisms that cause losses due to cold stress are not well-understood. To survey miRNAs and genes involved in cold tolerance, RNA-seq, miRNA-seq, and integration analyses were performed on . Results showed that a total of 118,015 genes and 6,034 of these differentially expressed genes (DEGs) were screened. Protein-protein interaction (PPI) analyses revealed that ABA signaling via protein phosphatase 2Cs was the most important signal transduction pathway and late embryogenesis abundant protein was the hub protein associated with adaptation to cold stress. Furthermore, a total of 856 miRNAs were identified in this study and 109 of them were differentially expressed in sugarcane responding to cold stress. Most importantly, the miRNA-gene regulatory networks suggested the complex post-transcriptional regulation in sugarcane under cold stress, including 10 miRNAs-42 genes, 16 miRNAs-70 genes, and three miRNAs-18 genes in CT vs. LT0.5, CT vs. LT1, and CT0.5 vs. LT1, respectively. Specifically, key regulators from 16 genes encoding laccase were targeted by novel-Chr4C_47059 and Novel-Chr4A_40498, while five genes were targeted by Novel-Chr6B_65233 and Novel-Chr5D_60023, 19 PPR repeat proteins by Novel-Chr5C_57213 and Novel-Chr5D_58065. Our findings suggested that these miRNAs and cell wall-related genes played vital regulatory roles in the responses of sugarcane to cold stress. Overall, the results of this study provide insights into the transcriptional and post-transcriptional regulatory network underlying the responses of sugarcane to cold stress.
冷胁迫给甘蔗生产造成了重大损失，然而，导致冷胁迫造成损失的确切分子机制尚未得到充分了解。为了研究参与耐寒性的miRNA和基因，对……进行了RNA测序、miRNA测序和整合分析。结果显示，共筛选出118,015个基因，其中有6,034个差异表达基因（DEG）。蛋白质-蛋白质相互作用（PPI）分析表明，通过蛋白磷酸酶2C的脱落酸信号传导是最重要的信号转导途径，晚期胚胎发生丰富蛋白是与适应冷胁迫相关的枢纽蛋白。此外，本研究共鉴定出856个miRNA，其中109个在甘蔗对冷胁迫的响应中差异表达。最重要的是，miRNA-基因调控网络表明了冷胁迫下甘蔗复杂的转录后调控，在CT与LT0.5、CT与LT1、CT0.5与LT1中分别包括10个miRNA-42个基因、16个miRNA-70个基因和3个miRNA-18个基因。具体而言，编码漆酶的16个基因中的关键调节因子被新型Chr4C_47059和新型Chr4A_40498靶向，5个基因被新型Chr6B_65233和新型Chr5D_60023靶向，19个PPR重复蛋白被新型Chr5C_57213和新型Chr5D_58065靶向。我们的研究结果表明，这些miRNA和细胞壁相关基因在甘蔗对冷胁迫的响应中发挥了重要的调控作用。总体而言，本研究结果为甘蔗对冷胁迫响应的转录和转录后调控网络提供了见解。
