Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
College of Agriculture, Guangxi University, Nanning 530005, China.
Genes (Basel). 2022 Jan 27;13(2):246. doi: 10.3390/genes13020246.
Sugarcan e is a major crop for sugar and biofuel production and is cultivated in tropical and subtropical areas worldwide. Sugarcane growth is constrained because of winter's low-temperature stress, and cold resistance is an important limitation in sugarcane growth enhancement. Therefore, in this study, we identified a gene involved in the low-temperature stress response of sugarcane. Calcineurin B-like (CBL) protein is a calcium signal receptor involved in the cold stress response. Five sugarcane CBL genes were cloned, sequenced, and named , , , , and . The protein sequences of these genes were analyzed. The calculated molecular weight of these proteins was 24.5, 25.9, 25.2, 25.6, and 26.3 kD, respectively. Subcellular localization analysis revealed that SoCBL1, SoCBL3, SoCBL6, and SoCBL9 were situated in the cytoplasm, while SoCBL5 was present in mitochondria. Secondary structure analysis showed that these five CBL proteins had similar secondary structures. Conserved domain analysis displayed that each sugarcane CBL protein contained three conserved EF domains. According to the self-expanding values of the phylogenetic tree, the CBL gene family was divided into four groups. The and genes were classified into one group, illustrating that these two genes might possess a similar function. The expression analysis of the gene under low temperatures showed that and were affected significantly, while and were less affected. These results demonstrate that the genes in sugarcane have similar characteristics and present differences in genetic diversity and gene expression response to low temperatures. Therefore, these genes might be novel candidates for fighting cold stress in sugarcane.
甘蔗是一种主要的糖料作物和生物燃料生产作物，在全球热带和亚热带地区都有种植。甘蔗的生长受到冬季低温胁迫的限制，而抗寒性是提高甘蔗生长的重要限制因素。因此，在本研究中，我们鉴定了一个与甘蔗低温胁迫反应相关的基因。钙调神经磷酸酶 B 样（CBL）蛋白是一种参与冷胁迫反应的钙信号受体。我们克隆、测序并命名了 5 个甘蔗 CBL 基因，分别为 、 、 、 、 。分析了这些基因的蛋白质序列。这些蛋白质的计算分子量分别为 24.5、25.9、25.2、25.6 和 26.3kD。亚细胞定位分析表明，SoCBL1、SoCBL3、SoCBL6 和 SoCBL9 位于细胞质中，而 SoCBL5 存在于线粒体中。二级结构分析表明，这 5 个 CBL 蛋白具有相似的二级结构。保守结构域分析显示，每个甘蔗 CBL 蛋白都含有三个保守的 EF 结构域。根据系统发育树的自展值，CBL 基因家族分为四个组。 和 基因被归为一组，表明这两个基因可能具有相似的功能。低温下 基因的表达分析表明， 和 受影响显著，而 和 受影响较小。这些结果表明，甘蔗中的 基因具有相似的特征，在遗传多样性和基因表达对低温的响应方面存在差异。因此，这些基因可能是甘蔗抗冷胁迫的新候选基因。
