实时扫描与图像分析。一种用于测定琼脂糖下中性粒细胞取向的快速方法。

Real-time scanning and image analysis. A fast method for the determination of neutrophil orientation under agarose.

作者信息

Pedersen J O, Hassing L, Grunnet N, Jersild C

机构信息

Regional Centre for Blood Transfusion and Clinical Immunology, Aalborg Hospital, Denmark.

出版信息

J Immunol Methods. 1988 Apr 22;109(1):131-7. doi: 10.1016/0022-1759(88)90450-4.

DOI:10.1016/0022-1759(88)90450-4

PMID:3356908

Abstract

We describe a newly developed method for fast determination of neutrophil chemotaxis and orientation in concentration gradients of chemotactic factors. The system implements video-based real-time scanning and image analysis of neutrophil migration under agarose, using an interactive easy-to-use computer program. Two methods for determining cell orientation are presented. No statistically significant difference between the methods was found. The analysis program distinguishes between chemokinetic and chemotactic behaviour of the cells (P less than 0.01).

摘要

我们描述了一种新开发的方法，用于快速测定趋化因子浓度梯度下中性粒细胞的趋化性和定向性。该系统通过一个交互式易用的计算机程序，对琼脂糖下中性粒细胞的迁移进行基于视频的实时扫描和图像分析。提出了两种确定细胞定向的方法。未发现这两种方法之间存在统计学上的显著差异。该分析程序能够区分细胞的化学动力学行为和趋化行为（P小于0.01）。