Zheng Sizhu, Li Yang, Yang Xiaojun, Chen Jingyun, Hua Jing, Gao Yuan
Suzhou entry exit inspection and Quarantine Bureau, Suzhou, Jiangsu Province, P.R. China.
Jiangsu Entry- Exit Inspection and Quarantine Bureau, Lab for plant quarantine, Nanjing, Jiangsu Province, P.R. China.
Mitochondrial DNA B Resour. 2018 Apr 4;3(1):419-423. doi: 10.1080/23802359.2018.1457988.
DNA barcoding is a recently developed technique for species-level identification that involves the use of short, standard DNA sequences as species labels. It is an effective complement to traditional taxonomic classification based on morphology. At present, research and applications involving the DNA barcoding of the Pseudococcidae are focused primarily on the cytochrome coxidase subunit I () gene, but there is not yet a consensus on the preferred gene region for barcoding. The purpose of this study was to explore the effectiveness of identification of Pseudococcidae beetles using DNA barcoding technology. The gene sequences of 97 samples from 21 species of Asemini were analysed, followed by evaluation of the ability to identify species using a tree-building method and distance evaluation. The sequences (500 bp) exhibited distinct distributions of intra-specific and inter-specific variation and a significant barcoding gap. The success rate of identification was 97.84%. These results demonstrate the feasibility of using this segment of to identify most species of Pseudococcidae.
DNA条形码技术是一种最近开发的用于物种水平鉴定的技术，它涉及使用短的标准DNA序列作为物种标签。它是基于形态学的传统分类学分类的有效补充。目前，涉及粉蚧科DNA条形码的研究和应用主要集中在细胞色素氧化酶亚基I（）基因上，但对于条形码的首选基因区域尚未达成共识。本研究的目的是探索使用DNA条形码技术鉴定粉蚧科甲虫的有效性。分析了来自21种长盾蚧属的97个样本的基因序列，然后使用建树方法和距离评估来评估鉴定物种的能力。序列（500bp）表现出明显的种内和种间变异分布以及显著的条形码间隙。鉴定成功率为97.84%。这些结果证明了使用该片段鉴定大多数粉蚧科物种的可行性。
