The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.
Mol Ecol Resour. 2012 Sep;12(5):791-6. doi: 10.1111/j.1755-0998.2012.03152.x. Epub 2012 May 2.
Ceroplastes Gray (wax scales) is one of the genera of Coccidae, most species of which are considered to be serious economic pests. However, identification of Ceroplastes species is always difficult owing to the shortage of easily distinguishable morphological characters. Mitochondrial cytochrome c oxidase I (COI) sequences (or DNA barcodes) and the D2 expansion segments of the large subunit ribosomal RNA gene 28S were used for accurate identification of six Ceroplastes species (C. floridensis Comstock, C. japonicus Green, C. ceriferus (Fabricius), C. pseudoceriferus Green, C. rubens Maskell and C. kunmingensis Tang et Xie) from 20 different locations in China. For COI data, low G·C content was found in all species, averaging about 20.4%. Sequence divergences (K2P) between congeneric species averaged 12.19%, while intra-specific divergences averaged 0.42%. All 112 samples fell into six reciprocally monophyletic clades in the COI neighbour-joining (NJ) tree. The NJ tree inferred from 28S showed almost same results, but samples of two closely related species, C. ceriferus and C. pseudoceriferus, were clustered together. This research indicates that the standard barcode region of COI can efficiently identify similar Ceroplastes species. This study provides an example of the usefulness of barcoding for Ceroplastes identification.
角蜡蚧属（蜡蚧）是盾蚧科的一个属，其中大多数物种被认为是严重的经济害虫。然而，由于缺乏易于区分的形态特征，角蜡蚧属物种的鉴定一直很困难。线粒体细胞色素 c 氧化酶 I（COI）序列（或 DNA 条码）和大亚基核糖体 RNA 基因 28S 的 D2 扩展片段被用于准确鉴定来自中国 20 个不同地点的 6 种角蜡蚧属物种（佛罗里达州角蜡蚧、日本角蜡蚧、日本角蜡蚧、假日本角蜡蚧、红角蜡蚧和昆明角蜡蚧）。对于 COI 数据，所有物种的 G·C 含量均较低，平均约为 20.4%。种间平均序列分歧（K2P）为 12.19%，而种内平均分歧为 0.42%。COI 邻接法（NJ）树中的 112 个样本分为 6 个相互单系的分支。28S 推断的 NJ 树几乎显示出相同的结果，但两种密切相关的物种，即日本角蜡蚧和假日本角蜡蚧的样本聚类在一起。本研究表明，COI 的标准条码区域可有效地鉴定相似的角蜡蚧属物种。本研究为角蜡蚧属鉴定的条形码应用提供了一个范例。
