Li Xianya, Wu Shunjiao, Xu Yonghong, Liu Yinghong, Wang Jia
College of Plant Protection, Southwest University, Chongqing 400715, China.
Insects. 2022 Mar 25;13(4):327. doi: 10.3390/insects13040327.
Frequent outbreaks have made Chlorops oryzae one of the major pests of rice in some regions. In order to understand the ecological adaptation of C. oryzae at the molecular level, and provide a scientific basis for formulating management strategies, we used two molecular markers, COI and ITS1 sequences, to systematically analyze the genetic structure of 31 populations. The higher haplotype diversity and lower nucleotide diversity indicated that the C. oryzae populations experienced rapid expansion after a “Bottleneck effect”. The results of the mismatch distribution, neutrality test (Fu’s Fs < 0, p < 0.001), and haplotype network analysis suggested that the population has recently undergone an expansion. Although genetic differentiation among C. oryzae populations was found to have existed at low/medium levels (Fst: 0.183 for COI, 0.065 for ITS1), the frequent gene flow presented as well (Nm: 2.23 for COI, 3.60 for ITS1) was supposed to be responsible for frequent local outbreaks.
频繁爆发使得稻秆蝇成为一些地区水稻的主要害虫之一。为了在分子水平上了解稻秆蝇的生态适应性，并为制定防治策略提供科学依据，我们使用了两个分子标记，即COI和ITS1序列，对31个种群的遗传结构进行了系统分析。较高的单倍型多样性和较低的核苷酸多样性表明，稻秆蝇种群在经历“瓶颈效应”后经历了快速扩张。失配分布、中性检验（Fu's Fs < 0，p < 0.001）和单倍型网络分析结果表明，该种群最近经历了一次扩张。尽管发现稻秆蝇种群之间存在低/中等水平的遗传分化（COI的Fst为0.183，ITS1的Fst为0.065），但频繁的基因流（COI的Nm为2.23，ITS1的Nm为3.60）也被认为是导致局部频繁爆发的原因。
