Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Am J Reprod Immunol. 2021 Jul;86(1):e13396. doi: 10.1111/aji.13396. Epub 2021 Feb 10.
Minimal evidence exists supporting therapeutic selections for equine placentitis. The goal of this study was to characterize the anti-inflammatory effects of firocoxib when administered to mares with placentitis.
Mares (gestation D270-300) were assigned to: INFECT (n = 6; placentitis, no treatment), FIRO (n = 6; placentitis, firocoxib, 0.1 mg/kg, PO, daily), and NORM (n = 6; no infection/treatment). Allantoic fluid (8 hours, 24 hours, birth) and amniotic fluid (birth) were collected from mares after infection. Concentrations of IL-1β, IL-6, TNF-α, IL-10, PGF , and PGE in fluids were measured by ELISA. mRNA expression of IL-1β, IL-6, TNF-α, IL-8, IL-10, matrix metalloproteinases (MMPs) -1, 3, and 9 in fetal membranes/fetuses was quantified using real-time PCR.
Allantoic TNF-α concentrations were lowest in FIRO at 8 hours and 24 hours post-infection; IL-6 concentrations were lower in FIRO than NORM at 8 hours, lower in FIRO than INFECT at 24 hours post-inoculation, and lower in NORM than FIRO or INFECT at birth. Marginal mean allantoic IL-β and IL-10 concentrations were lower in FIRO and NORM than INFECT. Amniotic fluid cytokines were lowest in NORM with all measurements in that group being below the limit of detection. Allantoic PGF concentrations were lower in FIRO and INFECT than NORM at 8 hours post-inoculation, and lower in FIRO than INFECT or NORM at 24 hours post-inoculation. Allantoic PGE concentrations were lower in FIRO than INFECT. Amniotic PGF and PGE concentrations were lower in NORM than INFECT. In fetal membranes, group differences with respect to IL-1β, IL-6, IL-8, and MMP1 were dependent on tissue type.
Data suggest a suppressive effect of firocoxib administration on cytokine and prostaglandin production in mares with placentitis.
支持马胎盘炎治疗选择的证据很少。本研究的目的是描述在患有胎盘炎的母马中给予非诺昔康的抗炎作用。
将妊娠第 270-300 天的母马分为以下三组：INFECT（n=6；胎盘炎，未治疗）、FIRO（n=6；胎盘炎，非诺昔康，0.1mg/kg，口服，每日）和 NORM（n=6；无感染/治疗）。感染后从母马收集羊水（8 小时、24 小时、分娩时）和羊水（分娩时）。通过 ELISA 测量液体中 IL-1β、IL-6、TNF-α、IL-10、PGF 和 PGE 的浓度。使用实时 PCR 定量胎儿膜/胎儿中 IL-1β、IL-6、TNF-α、IL-8、IL-10、基质金属蛋白酶（MMPs）-1、3 和 9 的 mRNA 表达。
感染后 8 小时和 24 小时，FIRO 的 TNF-α 浓度最低；FIRO 在 8 小时时的 IL-6 浓度低于 NORM，在 24 小时时的 IL-6 浓度低于 INFECT，在出生时的 IL-6 浓度低于 NORM 或 INFECT。FIRO 和 NORM 的 IL-β和 IL-10 浓度均低于 INFECT。在羊水细胞因子中，NORM 组的所有测量值均低于检测限。感染后 8 小时，FIRO 和 INFECT 的羊水 PGF 浓度均低于 NORM，24 小时时，FIRO 的羊水 PGF 浓度低于 INFECT 或 NORM。FIRO 的羊水 PGE 浓度低于 INFECT。NORM 的羊水 PGF 和 PGE 浓度均低于 INFECT。在胎膜中，IL-1β、IL-6、IL-8 和 MMP1 方面的组间差异取决于组织类型。
数据表明，非诺昔康给药对患有胎盘炎的母马细胞因子和前列腺素产生具有抑制作用。
