First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China.
Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
Pharm Biol. 2021 Dec;59(1):167-174. doi: 10.1080/13880209.2021.1878237.
The leech and centipede granules have good curative effects on many diabetic vascular diseases, including diabetes-induced erectile dysfunction (DIED).
To explore the effect of leech and centipede on erectile function in rats with diabetes-induced erectile dysfunction and its possible mechanism.
Thirty male Sprague-Dawley DIED rats were randomly divided into the model group (Group M), low-dose group (Group DD), high-dose group (Group DG) and tadalafil group (Group T) ( = 6); diabetic rats were induced by streptozotocin. Apomorphine was used to induce diabetic erectile dysfunction. The 'leech-centipede' granules (0.15 and 0.6 g/kg) were intragastrically administered in the DD and DG groups for 8 weeks. Blood glucose, serum insulin, testosterone, cGMP levels and protein expression changes were measured in each group.
After 8 weeks, the erectile function of rats in the DG group significantly improved (1.26 ± 0.73). Penis tissue cGMP levels were higher in the DG group (1.48 ± 0.11) than in the M group (0.58 ± 0.15). Protein and mRNA expression levels of NOS were significantly higher (0.77 ± 0.05; 0.61 ± 0.02) but those of PDE5 (0.43 ± 0.05; 0.61 ± 0.03) were lower in the DG group than in the M group (0.37 ± 0.06; 0.51 ± 0.01; 0.78 ± 0.06; 0.81 ± 0.04).
The leech-centipede can improve erectile dysfunction in DIED rats by regulating the expression of cGMP, NOS, and PDE5-related molecules in the PDE5 pathway. This study provides a potential mechanism for the treatment of DIED with leech-centipede.
水蛭和蜈蚣在许多糖尿病血管疾病方面具有良好的疗效，包括糖尿病性勃起功能障碍（DIED）。
探讨水蛭和蜈蚣对糖尿病性勃起功能障碍大鼠勃起功能的影响及其可能的机制。
30 只雄性 Sprague-Dawley DIED 大鼠随机分为模型组（M 组）、低剂量组（DD 组）、高剂量组（DG 组）和他达拉非组（T 组）（n=6）；通过链脲佐菌素诱导糖尿病大鼠，使用阿扑吗啡诱导糖尿病性勃起功能障碍。DD 组和 DG 组大鼠分别灌胃“水蛭-蜈蚣”颗粒（0.15 和 0.6g/kg）8 周。测量各组大鼠的血糖、血清胰岛素、睾酮、cGMP 水平和蛋白表达变化。
8 周后，DG 组大鼠的勃起功能显著改善（1.26±0.73）。DG 组阴茎组织 cGMP 水平（1.48±0.11）高于 M 组（0.58±0.15）。DG 组 NOS 的蛋白和 mRNA 表达水平（0.77±0.05；0.61±0.02）显著升高，但 PDE5 的蛋白和 mRNA 表达水平（0.43±0.05；0.61±0.03）则低于 M 组（0.37±0.06；0.51±0.01；0.78±0.06；0.81±0.04）。
水蛭-蜈蚣可通过调节 PDE5 通路中 cGMP、NOS 和 PDE5 相关分子的表达，改善 DIED 大鼠的勃起功能障碍。本研究为水蛭-蜈蚣治疗 DIED 提供了潜在的机制。
