Jülich Center for Neutron Science (JCNS-1) and Institute of Biological Information Processing (IBI-8) Forschungszentrum Jülich GmbH, 52428 Jülich, Germany.
Neutron Sciences Directorate, Oak Ridge National Laboratory (ORNL), POB 2008, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States.
Langmuir. 2021 Feb 23;37(7):2362-2375. doi: 10.1021/acs.langmuir.0c03302. Epub 2021 Feb 11.
We investigated the influence of an -alkyl-PEO polymer on the structure and dynamics of phospholipid vesicles. Multilayer formation and about a 9% increase in the size in vesicles were observed by cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), and small-angle neutron/X-ray scattering (SANS/SAXS). The results indicate a change in the lamellar structure of the vesicles by a partial disruption caused by polymer chains, which seems to correlate with about a 30% reduction in bending rigidity per unit bilayer, as revealed by neutron spin echo (NSE) spectroscopy. Also, a strong change in lipid tail relaxation was observed. Our results point to opportunities using synthetic polymers to control the structure and dynamics of membranes, with possible applications in technical materials and also in drug and nutraceutical delivery.
我们研究了 - 烷氧基聚氧乙烯聚合物对磷脂囊泡结构和动力学的影响。低温透射电子显微镜（cryo-TEM）、动态光散射（DLS）和小角中子/ X 射线散射（SANS/SAXS）观察到多层形成和囊泡尺寸增加约 9%。结果表明，聚合物链部分破坏导致囊泡的层状结构发生变化，这似乎与每单位双层的弯曲刚度降低约 30%相关，这是通过中子自旋回波（NSE）光谱揭示的。此外，还观察到脂质尾部弛豫的强烈变化。我们的结果表明，使用合成聚合物来控制膜的结构和动力学具有可能性，这可能在技术材料以及药物和营养保健品的输送方面具有应用前景。
