College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; Xiamen Engineering and Technological Research Center for Comprehensive Utilization of Marine Biological Resources, Xiamen 361021, China.
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
Food Res Int. 2021 Jun;144:110360. doi: 10.1016/j.foodres.2021.110360. Epub 2021 Apr 5.
Obesity is regarded to be associated with fat accumulation, chronic inflammation, and gut microbiota dysbiosis. Raw and ripened pu-erh tea extract (PETe) have the effect of reducing body weight gain and fat accumulation, which are associated with gut microbiota. However, little is known about the difference of raw and ripened PETe on the regulation of gut microbiota. Here, our results suggested that supplementation of raw and ripened PETe displayed similar anti-obesogenic effect in high fat diet (HFD)-induced obesity mice, by attenuating the body weight gain, fat accumulation, oxidative injury, and low-grade inflammation, improving the glucose tolerance, alleviating the metabolic endotoxemia, and regulating the mRNA and protein expression levels of the lipid metabolism-related genes. 16S rRNA sequencing of fecal samples indicated that raw and ripened PETe intervention displayed different regulatory effect on the HFD-induced gut microbiota dysbiosis at different taxonomic levels. The microbial diversity, the relative abundance of Firmicutes and Bacteroidetes as well as F/B ratio were reversed more closer to normal by ripened PETe. Phylotypes of Bacteroidaceae, Ruminococcaceae, Lachnospiraceae, Muribaculaceae, and Rikenellaceae which are negatively correlated with obesity were enhanced notably by the intervention of ripened PETe, while Erysipelotrichaceae and Lactobacillaceae which have positive correlation with obesity were decreased dramatically. In addition, the treatment of ripened PETe had better effect on the increase of benefical Bacteroides, Alistipes, and Akkemansia and decrease of obesity associated Faecalibaculum and Erysipelatoclostridium (p < 0.05). These findings suggested that pu-erh tea especially ripened pu-erh tea could serve as a great candidate for alleviation of obesity in association with the modulation of gut microbiota.
肥胖被认为与脂肪积累、慢性炎症和肠道微生物群落失调有关。生茶和熟茶普洱提取物（PETe）具有减轻体重增加和脂肪积累的作用，这与肠道微生物群落有关。然而，人们对生茶和熟茶对肠道微生物群落的调节作用的差异知之甚少。在这里，我们的研究结果表明，生茶和熟茶 PETe 的补充在高脂肪饮食（HFD）诱导的肥胖小鼠中表现出相似的抗肥胖作用，通过减轻体重增加、脂肪积累、氧化损伤和低度炎症，改善葡萄糖耐量，缓解代谢性内毒素血症，并调节脂质代谢相关基因的 mRNA 和蛋白质表达水平。粪便样本的 16S rRNA 测序表明，生茶和熟茶 PETe 干预对 HFD 诱导的肠道微生物群落失调在不同分类水平上表现出不同的调节作用。微生物多样性、厚壁菌门和拟杆菌门的相对丰度以及 F/B 比值通过熟茶 PETe 的干预更接近正常水平得到逆转。与肥胖呈负相关的拟杆菌科、瘤胃球菌科、毛螺菌科、穆里布菌科和理研菌科的菌群丰度显著增强，而与肥胖呈正相关的肠球菌科和乳杆菌科则显著减少。此外，熟茶 PETe 的处理对有益的拟杆菌、阿克曼氏菌和 Akkemansia 的增加以及肥胖相关的粪杆菌和产芽孢梭菌的减少有更好的效果（p<0.05）。这些发现表明，普洱茶特别是熟茶可能是缓解肥胖的候选药物，与调节肠道微生物群落有关。
