Adra Cherrihan, Panchalingam Harrchun, Foster Keith, Tomlin Russell, Hayes R Andrew, Kurtböke D İpek
School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, QLD, Australia.
Brisbane City Council, Program, Planning and Integration, Brisbane Square, Brisbane, QLD, Australia.
Front Plant Sci. 2024 Mar 4;15:1371285. doi: 10.3389/fpls.2024.1371285. eCollection 2024.
is a plant pathogen that causes economic losses in agricultural and forestry industries, including significant destruction to amenity trees within the city of Brisbane in Australia. Use of chemical control agents are restricted in public areas, there is therefore an urgent need to investigate biological control approaches. Members of the phylum Actinomycetota, commonly known as actinomycetes, are known for their industrially important secondary metabolites including antifungal agents. They have proven to be ideal candidates to produce environmentally friendly compounds including the volatile organic compounds (VOCs) which can be used as biofumigants.
Different species (n=15) previously isolated from the guts of termites and stored in the University of the Sunshine Coast'sMicrobial Library were tested for their antifungal VOCs against .
Fourteen of them were found to display inhibition (39.39-100%) to the mycelial development of the pathogen. Strongest antifungal activity displaying isolates USC-592, USC-595, USC-6910 and USC-6928 against the pathogen were selected for further investigations. Their VOCs were also found to have plant growth promotional activity observed for with an increase of root length (22-36%) and shoot length (26-57%). The chlorophyll content of the test plant had a slight increase of 11.8% as well. Identified VOCs included geosmin, 2-methylisoborneol, 2-methylbutyrate, methylene cyclopentane, β-pinene, dimethyl disulfide, ethyl isovalerate, methoxyphenyl-oxime and α-pinene. Additionally, all 15 Streptomyces isolates were found to produce siderophores and indole acetic acid as well as the enzyme chitinase which is known to break down the fungal cell wall.
Findings indicate that termite gut-associated streptomycetes might be used to control by utilizing their wide range of inhibitory mechanisms.
是一种植物病原体，会给农业和林业产业造成经济损失，包括对澳大利亚布里斯班市内的观赏树木造成严重破坏。在公共区域，化学防治剂的使用受到限制，因此迫切需要研究生物防治方法。放线菌门的成员，通常被称为放线菌，以其具有重要工业价值的次级代谢产物而闻名，包括抗真菌剂。事实证明，它们是生产环保化合物的理想候选者，包括可作为生物熏蒸剂的挥发性有机化合物（VOCs）。
对先前从白蚁肠道中分离并保存在阳光海岸大学微生物库中的不同物种（n = 15）进行测试，检测其对的抗真菌挥发性有机化合物。
发现其中14种对病原体的菌丝发育表现出抑制作用（39.39 - 100%）。选择对病原体表现出最强抗真菌活性的分离株USC - 592、USC - 595、USC - 6910和USC - 6928进行进一步研究。还发现它们的挥发性有机化合物具有促进植物生长的活性，观察到根长增加（22 - 36%），茎长增加（26 - 57%）。受试植物的叶绿素含量也略有增加，为11.8%。鉴定出的挥发性有机化合物包括土臭素、2 - 甲基异冰片、2 - 甲基丁酸酯、亚甲基环戊烷、β - 蒎烯、二甲基二硫、异戊酸乙酯、甲氧基苯基肟和α - 蒎烯。此外，发现所有15株链霉菌分离株都能产生铁载体、吲哚乙酸以及已知可分解真菌细胞壁的几丁质酶。
研究结果表明，与白蚁肠道相关的链霉菌可能通过利用其广泛的抑制机制来控制病原体。
