Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
J Appl Microbiol. 2021 Sep;131(3):1515-1530. doi: 10.1111/jam.15038. Epub 2021 Mar 2.
To reveal the sources of Aeromonas infection in Okinawa Prefecture of Japan, the species, virulence genes and clones of strains isolated from clinical specimens and well water were compared.
The properties of both isolates were investigated by sequencing of rpoD, detection of 10 virulence genes using PCR and genotyping with pulsed-field gel electrophoresis. In all, 68 clinical and 146 well water strains of Aeromonas were isolated and the main species were A. caviae, A. dhakensis, A. hydrophila and A. veronii biovar sobria. Aeromonas dhakensis possessed various virulence genes; however, A. caviae possessed only fla. The same or similar clones were distributed in certain areas of Okinawa and one clone had survived several months in the biliary system of two patients, respectively.
Although the same Aeromonas clone was not isolated from clinical and well water samples, our study revealed the detected patterns of virulence genes in both isolates, the distribution of identical/similar clones in the Okinawan environment and long-time survival in patient's organs.
We investigated the association between Aeromonas patients and well water exposure. This study provides the properties of species, virulence genes and clones of Aeromonas isolated from samples of these origins.
为了揭示日本冲绳地区气单胞菌感染的来源，比较了从临床标本和井水分离的菌株的种属、毒力基因和克隆。
通过 rpoD 测序、10 种毒力基因的 PCR 检测和脉冲场凝胶电泳基因分型，研究了分离株的特性。共分离出 68 株临床菌株和 146 株井水菌株，主要种属为豚鼠气单胞菌、达卡气单胞菌、嗜水气单胞菌和温和气单胞菌。达卡气单胞菌具有多种毒力基因，而豚鼠气单胞菌仅具有 fla 基因。在冲绳的某些地区分布着相同或相似的克隆，其中一个克隆在两名患者的胆道系统中分别存活了几个月。
尽管未从临床和井水样本中分离出相同的气单胞菌克隆，但本研究揭示了两株分离株中检测到的毒力基因模式、冲绳环境中相同/相似克隆的分布以及在患者器官中的长时间存活。
我们调查了气单胞菌患者与井水暴露之间的关联。本研究提供了来源于这些来源的气单胞菌分离株的种属、毒力基因和克隆的特性。
