Elimination of Multidrug-Resistant Bacteria by Transition Metal Dichalcogenides Encapsulated by Synthetic Single-Stranded DNA.

Antibiotic-resistant bacteria are a significant and growing threat to human health. Recently, two-dimensional (2D) nanomaterials have shown antimicrobial activity and have the potential to be used as new approaches to treating antibiotic resistant bacteria. In this Research Article, we exfoliate transition metal dichalcogenide (TMDC) nanosheets using synthetic single-stranded DNA (ssDNA) sequences, and demonstrate the broad-spectrum antibacterial activity of MoSe encapsulated by the T ssDNA sequence in eliminating several multidrug-resistant (MDR) bacteria. The MoSe/T is able to eradicate Gram-positive and Gram-positive at much lower concentrations than graphene-based nanomaterials. Eradication of MDR strains of methicillin-resistant (MRSA), , , , and are shown to occur at at 75 μg mL concentration of MoSe/T, and at 150 μg mL. Molecular dynamics simulations show that the thymine bases in the T sequence lie flat on the MoSe surface and can, thus, form a very good conformal coating and allow the MoSe to act as a sharp nanoknife. Electron microscopy shows the MoSe nanosheets cutting through the cell membranes, resulting in significant cellular damage and the formation of interior voids. Further assays show the change in membrane potential and reactive oxygen species (ROS) formation as mechanisms of antimicrobial activity of MoSe/T. The cellular death pathways are also examined by mRNA expression. This work shows that biocompatible TMDCs, specifically MoSe/T, is a potent antimicrobial agent against MDR bacteria and has potential for clinical settings.