Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
Department of Chemical Engineering, Institute of Chemical Technology, Mumbai 400019, India.
Ultrason Sonochem. 2021 May;73:105478. doi: 10.1016/j.ultsonch.2021.105478. Epub 2021 Feb 2.
The present study deals with intensified synthesis of designer lipids with application of ultrasound based on biocatalyzed reaction between long chain triglyceride and medium chain fatty acid. The effects of various reaction conditions like molar ratio of reactant, reaction temperature, and enzyme loading along with the effect of ultrasound parameters such as duty cycle and irradiation time on the rate of formation of designer lipids has been investigated. The ultrasound assisted process was also compared with the traditional process so as to clearly bring out the intensification effects. During the study, it was clearly demonstrated that the optimum reaction conditions for maximum yield of designer lipids as 92% was molar ratio of medium chain fatty acid to long chain triglyceride as 4:1, reaction temperature of 40 °C, enzyme loading of 3%, duty cycle of 70%, 240 W as power dissipation and 360 min as reaction time. The recyclability study of enzyme showed its effectiveness up to 10 cycles. The synthesized designer lipid showed higher oxidative stability for 35 days and also showed Newtonian behaviour with eye appealing colour. The current study demonstrates development of an eco-friendly technique for intensified synthesis of designer lipids having numerous nutraceutical benefits.
本研究采用超声强化生物催化长链甘油三酯与中链脂肪酸反应，合成设计脂质。考察了反应物摩尔比、反应温度、酶用量等各种反应条件以及占空比、辐照时间等超声参数对设计脂质生成速率的影响，并将超声辅助法与传统法进行了比较，以清楚地显示强化效果。研究表明，最大产率为 92%的最佳反应条件为中链脂肪酸与长链甘油三酯摩尔比为 4:1、反应温度为 40°C、酶用量为 3%、占空比为 70%、功率耗散为 240 W、反应时间为 360 min。酶的循环使用研究表明，其有效性可达 10 次。合成的设计脂质具有较高的 35 天氧化稳定性，且颜色美观，呈牛顿流体行为。本研究开发了一种环保的强化设计脂质合成技术，具有多种营养益处。
