Ward R L, Knowlton D R, Schiff G M, Hoshino Y, Greenberg H B
James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219.
J Virol. 1988 May;62(5):1543-9. doi: 10.1128/JVI.62.5.1543-1549.1988.
Two outer capsid rotavirus proteins, VP3 and VP7, have been found to elicit neutralizing-antibody production, but the immunogenicity of these proteins during human rotavirus infection has not been determined. The relative amounts of serum neutralizing antibody against the VP3 and VP7 proteins of the CJN strain of human rotavirus were, therefore, determined in adult subjects before and after infection with this virus. Reassortant strains of rotavirus that contained the CJN gene segment for only one of these two neutralization proteins were isolated and used for this study. The geometric mean titer of serum neutralizing antibody to a reassortant virus (CJN-M) that contained VP7 of CJN and VP3 of another human rotavirus was 12.7 times less than that of antibody to CJN before infection and 20.3 times less after infection. This indicated that most neutralizing antibody was against the VP3 rather than the VP7 protein of CJN. This result was confirmed with other reassortants between CJN and animal rotavirus strains (EDIM and rhesus rotavirus). These findings suggest that VP3 is the primary immunogen that stimulates neutralizing antibody during at least some rotavirus infections of humans.
已发现两种轮状病毒外衣壳蛋白VP3和VP7可引发中和抗体产生，但这些蛋白在人类轮状病毒感染期间的免疫原性尚未确定。因此，在成年受试者感染人类轮状病毒CJN株之前和之后，测定了针对该病毒VP3和VP7蛋白的血清中和抗体的相对量。分离出仅含有这两种中和蛋白之一的CJN基因片段的轮状病毒重配株并用于本研究。针对含有CJN的VP7和另一种人类轮状病毒的VP3的重配病毒（CJN-M）的血清中和抗体几何平均滴度在感染前比针对CJN的抗体低12.7倍，感染后低20.3倍。这表明大多数中和抗体针对的是CJN的VP3而非VP7蛋白。CJN与动物轮状病毒株（EDIM和恒河猴轮状病毒）之间的其他重配株也证实了这一结果。这些发现表明，VP3是在人类至少某些轮状病毒感染期间刺激中和抗体产生的主要免疫原。
